摘要
为了探究不同浓度外源脱落酸(ABA)对盐碱胁迫下苜蓿幼苗内源激素含量及激素比值的影响,试验以紫花苜蓿WL343HQ为试验材料,对150 mmol/L苏打盐碱胁迫下苜蓿幼苗喷施25,50,75,100μmol/L的ABA溶液,采用HPLC法对苜蓿幼苗的根和叶吲哚乙酸(IAA)、赤霉素(GA3)和ABA含量进行测定。结果表明:随着外源ABA浓度的升高,IAA和GA3含量呈逐渐下降的趋势,ABA呈逐渐上升的趋势,IAA/ABA、GA3/ABA比值均呈逐渐下降的趋势,且ABA处理显著影响了3种激素的含量和比值的变化,当ABA处理浓度超过50μmol/L时,叶中3种激素含量及比值均大于根。说明外源ABA对苏打盐碱胁迫下苜蓿幼苗根和叶中IAA、GA3、ABA激素含量的变化对调控苜蓿抗盐碱方面发挥着重要的作用。
In order to explore the effect of different concentration exogenous abscisic acid(ABA)on endogenous hormone content and hormone ratio of alfalfa seedlings under salt and alkali stress,Medicago sativa L.WL343 HQ was used as experimental material in this experiment,25,50,75 and 100μmol·L^-1 ABA solution were used to spray alfalfa seedlings under 150 mmoL·L^-1 soda alkali stress.The contents of IAA,GA3 and ABA in roots and leaves of alfalfa seedlings were determined by high performance liquid chromatography(HPLC).The results showed that with the increase of exogenous ABA concentration,the contents of IAA and GA3 decreased gradually,ABA content increased gradually,and the ratios of IAA/ABA and GA3/ABA decreased gradually,suggesting that ABA treatment significantly affected the contents and ratios of the three hormones.When ABA concentration was more than 50μmol·L^-1,the content and ratio of three hormones in leaves were higher than those in roots.The results indicated that exogenous ABA played an important role in the regulation of saline-alkali resistance of alfalfa by regulating the contents of IAA,GA3,ABA hormone in roots and leaves of alfalfa seedlings under soda alkali stress.
作者
李红
李波
杨曌
LI Hong;LI Bo;YANG Zhao(Institute of Animal Husbandr>r and Veterinary of Heilongjiang academy of Agricultural sciences,Qiqihar 161005;College of Agriculture,Forestry and Life Sciences/Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2020年第6期103-106,111,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
现代农业产业技术体系建设专项资金项目(CARS-34)
黑龙江省省属高等学校基本科研业务费科研项目(135209267,YSTSXK201886)
齐齐哈尔市科技计划项目(NYGG-201916)
齐齐哈尔大学研究生创新科研项目(YJSCX2019043)。
