摘要
采用恒温水浴振荡方法提取杏鲍菇水溶性粗多糖,再经分离纯化得固体杏鲍菇多糖。以对氧自由基的清除率为评价指标,采用邻苯三酚自氧化法和邻二氮菲-Fe2+-H2O2氧化法研究了杏鲍菇多糖对氧自由基的清除能力。结果表明:杏鲍菇多糖对羟自由基和超氧阴离子自由基均有清除作用。在一定的多糖浓度范围内随着多糖浓度的增加对羟自由基和超氧阴离子自由基的清除作用亦增强,并呈良好的线性关系;杏鲍菇多糖浓度在0.7mg/mL对羟自由基的清除率可达98.65%,对超氧阴离子自由基的清除率可达37.66%。
Soluble crude polysaccharide form Pleurotus eryngii were extracted by the method of constant temperature water bath shaking,and solid polysaccharide of Pleurotus eryngii was isolated and purified.Using scavenging percentage of polysaccharide of Pleurotus eryngii to oxygen free radical as index,scavenging activity was determined with pyrogallol autoxidation method and phenanthroline-Fe2＋ reaction system.The results showed that polysaccharide of Pleurotus eryngii had significant effect on scavenging hydroxyl radicals and super oxide anion radicals.The scavenging activity of polysaccharide of Pleurotus eryngii clearance both superoxide anion radicaland hydroxyl radical increased with the rise of polysaccharide concentration at a certain range,and there was a good linear relationship.When the concentration of polysaccharide was 0.7 mg/mL,the percentage of scavenging hydroxyl radicals could reach 98.65%,and the percentage of scavenging super oxide anion radicals was 37.66%.
出处
《北方园艺》
CAS
北大核心
2013年第11期146-148,共3页
Northern Horticulture
关键词
杏鲍菇
多糖
抗氧化作用
Pleurotus eryngii
polysaccharide
antioxidant effect
