摘要
目的考察EPO-α-MHC启动子在缺氧条件下的调控表达作用。方法构建pGL3-EPO-α-MHC启动子编码荧光素酶报告基因的质粒,以含pGL3-EPO-α-MHC和pGL3-CMV启动子的质粒分别转染缺氧和常氧的心肌细胞(H9C2),检测荧光素酶的表达。结果 pGL3-CMV启动子质粒在缺氧条件下的报告基因表达与常氧条件相比显著下降(P<0.05),而pGL3-EPO-α-MHC启动子在缺氧条件下能够显著上调报告基因的表达4.3倍(P<0.01)。结论 EPO-α-MHC启动子具有缺氧调控功能。
Objective To investigate the expression regulated function of EPO-α-MHC promoter under hypoxic conditions. Methods pGL3-EPO-α-MHC-luciferase plasmid was constructed. Hypoxic and normoxic cardiomyocytes （H9C2） were transfected by plasmids with pGL3-EPO-α-MHC-luciferase and pGL3-CMV-luciferase plasmids, respectively. The expression of luciferase in cardiomyocytes were detected. Results The luciferase expression of plasmids with pGL3-CMV promoter in hypoxic conditions was significantly decreased （P 〈 0.05） compare with that in normoxic conditions. The expression of report gene with pGL3-EPO-α-MHC promoter was significantly up-regulated 4.3 times under hypoxic conditions （P 〈 0.01）. Conclusion EPO-α-MHC promoter has hypoxic regulation function.
出处
《中国当代医药》
2013年第16期8-9,12,共3页
China Modern Medicine
基金
国家自然科学基金(30901863)
