摘要
目的观察人血管内皮生长因子-B(human vascular endothelial cell growth factor-B,hVEGF-B)裸质粒DNA直接心肌注射对大鼠急性心肌缺血后心功能的影响。方法选用Wistar雄性大鼠50只,随机分为治疗组和对照组各25只。建立大鼠左冠状动脉结扎的急性心肌缺血模型,取缺血边缘区以先期构建的真核表达质粒pcDNA 3.1/hVEGF-B和真核载体pcDNA3.1的DNA分别行多点心肌注射。治疗组每点注射溶于生理盐水10μL中pcDNA3.1/hVEGF-B质粒DNA 10μg,对照组给予等量pcDNA3.1质粒DNA。4周后行血流动力学测定,检测梗死区血管数目及心肌细胞凋亡。结果给药后4周,治疗组血流动力学恢复较好,新生血管数目明显多于对照组(P<0.05);治疗组hVEGF-B表达较对照组明显增强(P<0.05);心肌细胞凋亡数量较对照组减少(P<0.05)。结论 pcDNA3.1/hVEGF-B裸质粒DNA心肌注射可转染心肌细胞并持续表达4周以上,可促进缺血心肌心功能恢复及血管新生。
Objective To investigate the effect of direct gene transfer of nacked DNA encoding human vascular endothelial growth factor-B on the cardiac function in rats with myocardial ischemia. Methods Fifty male Wistar rats were randomly divided into treatment group and control group, with 25 rats in each group. The rat models of acute myocardial ischemia were established by ligating left main coronary artery. The constructed eukaryotic expression plasmid pcDNA 3.1/hVEGF-B in advance and eukaryotic vector pcDNA3.1 DNA were injected into myocardium at multiple points around the ischemic border zone respectively, pcDNA3. 1/hVEGF-B plasmid DNA in dose of 10 /μg dissolved in 10 /μL saline was injected into each point in treatment group, and the same amount of pcDNA3. 1 plasmid DNA was given in control group. After 4 weeks, the cardiac function, the number of vessels in infarction area and the number of myocardial cell apoptosis were examined. Results Compared with control group, the hemodynamic indexes of rats recovered better after 4 weeks, the neovascularization number of rats increased significantly （P〈 0.05）, the expression intensity of hVEGF-B enhanced significantly （P〈0.05）, and the myocardial cell apoptosis number decreased （P〈0.05） in treatment group. Conclusion pcDNA3.1/hVEGF B can be intramyocardial transfected and continues to secrete bioactivity protein at least for 4 weeks, and can improve cardiac function and promote angiogenesis in ischemic myocardium.
出处
《中华实用诊断与治疗杂志》
2012年第12期1195-1197,共3页
Journal of Chinese Practical Diagnosis and Therapy
关键词
心肌缺血
血管内皮生长因子
基因疗法
侧支循环
Myocardial ischemia
vascular endothelial growth factor
gene therapy
collateral circulation
