摘要
目的:研究罗汉果提取物(SGFE)的抗运动疲劳作用,为SGFE的临床应用提供依据。方法:将144只雄性ICR小鼠随机分为对照组、SGFE低剂量组、SGFE中剂量组和SGFE高剂量组,每组36只。对照组小鼠每天口服1.0 mL生理盐水,其他3组小鼠每天给予1.0 mL SGFE(SGFE分别为100、200和400mg.kg-1),所有小鼠每周进行3次持续15min的被动游泳训练,持续28d。末次小鼠被动游泳实验后处死,采集血液、肝脏和腓肠肌分别检测小鼠血乳酸(BLA)、血尿素氮(BUN)、肝糖原和肌糖原浓度。结果:与对照组比较,SGFE低、中和高剂量3组小鼠的游泳力竭时间延长(P<0.05),但SGFE 3组间比较差异无统计学意义(P<0.05);SGFE低、中和高剂量组小鼠BLA浓度明显低于对照组(P<0.05),且随SGFE剂量增加有下降趋势;SGFE中剂量组和高剂量组小鼠BUN浓度明显低于对照组(P<0.05),但低剂量组小鼠BUN浓度与对照组比较差异无统计学意义。SGFE低、中和高剂量组小鼠肝糖原和肌糖原浓度明显高于对照组(P<0.05),且随着SGFE剂量的增加有升高趋势。结论:补充SGFE对运动疲劳小鼠有明显的抗疲劳功效。
Objective To discuss the anti-fatigue effects of siraitia grosvenorii fruit extracts（SGFE） on the mice with exercise fatigue and to provide basis for clinical application of SGFE.Methods 144 male ICR mice were randomly divided into control group（n=36）,SGFE low dosage group（n=36）,SGFE middle dosage group（n=36） and SGFE high dosage group（n=36）.The mice in control group were received an oral administration of physiological saline in a volume of 1.0 mL,and the mice in other three groups were received 1.0 mL of SGFE（100,200 and 400 mg·kg-1,once a day） for 28 d.All mice were forced to swim for 15 min each day.After 28 d,the blood,liver and gastrocnemius were collected after forced swimming test,and the biochemical parameters related to fatigue,including blood lactic acid（BLA）,blood urea nitrogen（BUN）,liver glycogen,and muscle glycogen were measured.Results Compared with control group,the exhautive swimming time of the mice in SGFE low,middle and high dosage groups was prolonged（P0.05）,but there were no significant differences between SGFE low dosage,middle dosage and high dosage groups（P0.05）;the levels of BLA of mice in SGFE low dosage,middle dosage and high dosage groups were significantly lower than that in control group（P0.05）,and the BLA levels showed a decreasing trend with the increase of SGFE in SGFE low,middle and high dosage groups.The levels of BUN of mice in SGFE middle and high dosage groups were significantly lower than that in control group（P0.05）,but there was no significant difference between SGFE low dosage group and control group.The levels of liver glycogen and muscle glycogen of mice in SGFE low,middle and high dosage groups were significantly higher than that in control group（P0.05）,and they showed a increasing trend with the increase of SGFE dosagein SGFE low,middle and high dosage groups.Conclusion SGFE has significant anti-fatigue effect on the mice with exercise fatigue.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第3期494-497,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅自然科学基金资助课题(20070573)
关键词
罗汉果提取物
运动疲劳
血乳酸
血尿素氮
小鼠
ICR
siraitia grosvenorii fruit extracts
physical fatigue
blood lactic acid
blood urea nitrogen
mice
ICR
