摘要
目的观察人胃癌细胞原位移植转移裸鼠模型原发灶及转移灶中锌指蛋白139(ZNF139)的表达,探讨其与肿瘤侵袭转移的关系。方法建立人胃癌细胞株SGC7901原位移植转移裸鼠模型,采用逆转录-聚合酶链反应、免疫组织化学S-P法检测ZNF139、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-7(MMP-7)在原发灶、肝转移灶、脾转移灶、腹膜种植转移灶、转移淋巴结中的表达情况。结果人胃癌细胞原位移植转移裸鼠模型成瘤率为93.3%(28/30),其中75.0%(21/28)发生肝转移、67.9%(19/28)发生脾转移、85.7%(24/28)发生腹膜种植转移、39.3%(11/28)发生淋巴结转移。ZNF139、MMP-2、MMP-7在肝转移灶、腹膜种植转移灶及转移淋巴结中mRNA表达水平和蛋白表达阳性率均高于原发灶(P<0.05),原发灶、各转移灶中ZNF139 mRNA与MMP-2 mR-NA、MMP-7 mRNA的表达均呈正相关(P<0.05)。结论人胃癌细胞原位移植转移裸鼠模型可很好地模拟胃癌在人体内的侵袭、转移过程。与原发灶比较,转移灶中肿瘤细胞具有更强的侵袭能力,可能与转移过程中ZNF139促进MMP-2、MMP-7的表达有关。
Objective To investigate the expression of zinc finger protein 139 （ZNF139） and its relationship with in- vasion and metastasis in the primary tumor and metastases of orthotopic implantation nude mice. Methods Orthotopic implanta- tion nude mice with human gastric carcinoma cells were established. Then reverse transcription - polymerase chain reaction （RT- PCR） and immunohistochemical method （S -P） were used to detect the expression of ZNF139, MMP- 2 and MMP- 7 in orthotopic transplantation tumor, liver metastases, spleen metastasis, peritoneal metastasis and lymph node metasta- sis. Results The tumor formation rate was 93.3% （28/30）, and hepatic metastases were 75.0% （21/28）, spleen metastases were 67. 9% （19/28）, peritoneal metastases were 85.7% （24/28） and lymph node metastases were 39. 3% （11/28） . The mRNA expression and protein positive expression rate of ZNF139, MMP - 2 and MMP - 7 in liver metastases, peritoneal metasta- ses and lymph node metastases were significantly higher than in primary tumor （ P 〈 0.05 ） . The expression of ZNF139 mRNA was positively correlated with the expression of MMP - 2 mRNA and MMP - 7 mRNA both in primary tumor and metastases （P 〈 O. 05）. Conclusion Models of orthotopic implantation nude mice can well simulate the invasion and transfer process. Invasion and metastasis are stronger in metastases than in primary tumor, and ZNF139 may be involved in this process by promoting the ex- pression of MMP -2 and MMP -7.
出处
《中国全科医学》
CAS
CSCD
北大核心
2013年第15期1740-1743,共4页
Chinese General Practice
基金
国家自然科学基金资助项目(81072033)
河北省自然科学基金资助项目(C2010000619)
河北省科技厅科技支撑项目(11276103D-26)
河北省普通高校强势特色学科资助项目(冀教高[2005]52)
关键词
胃肿瘤
原位移植模型
裸鼠
锌指蛋白139
侵袭
肿瘤
转移
Stomach neoplasms
Orthopotic implantation model, nude mice
Zinc finger protein 139
Invasiveness, neoplasms
Metastasis
