摘要
目的通过煅烧牙齿研究一种新型骨缺损修复材料——牙源性双相陶瓷生物骨,并进行骨缺损修复的动物实验。方法实验选用人的离体牙齿,经过高温煅烧去除有机成分后再次与磷酸氢二铵液体反应,经煅烧制成牙源性双相陶瓷生物骨,作为实验材料,使用X线衍射和扫描电镜观察材料的微观结构。动物实验选用新西兰大白兔,在其胫骨处制造骨缺损,分别填入实验材料或自体髂骨,以及不填入任何材料,并于4、8、12周处死,对样本行大体观察、影像学观察、组织形态学检测,初步评价该材料对骨缺损的修复效果。结果离体牙煅烧后不含有机物质,主要成分为羟基磷灰石/β-磷酸三钙(HA/β-TCP)。12周时,实验组骨缺损处可见新生骨生成,骨缺损能够基本修复,但仍残留未降解材料。结论牙源性双相陶瓷材料具有良好修复骨缺损的效果,其安全性需进一步验证。
Objective To investigate a method for preparing a new bone plant material-odontogenic biphasic ceramic-like biologic bone （BCBB）. Methods The detachment teeth were calcinated first time to remove the organic principle,and second calcinated with the aqua of （NH4 ）2HP04 ,then get odontogenic BCBB. At the same time, a series of examinations were carried out including scanning electron microscope （SEM） , energy dispersion analysis of X-ray. A shin bone defect was made in each rabbit. Three different kinds of bone grafts were implanted respectively：no grafts, autograft cancellous bone and odontogenic BCBB. 4,8 and 12 weeks after implantation, the rabbits were killed, the specimens were examined by X-ray and HE histological observation. Results No organic matter was found and the main composition of the calcinated teeth was HA/β-TCP. After 12 weeks, odontogenic BCBB was almost degraded, newly regenerated bone grew into and merged with the material and non-specific inflammation was found in the tissue around the implants. Conclusion Odontogenic BCBB could get good bone reconstruction. It may become a new transplantation material in clinical bone defect treatment if its safety character could be further studied.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第6期617-620,共4页
Acta Universitatis Medicinalis Anhui
关键词
双相陶瓷生物骨
骨缺损
成骨作用
biphasic ceramic-like biologic bone
bone defect
osteogenesis
