摘要
目的探讨多发性骨髓瘤（MM）U266细胞株和MM患者的骨髓单个核细胞（BMMNC）中DNA甲基化相关蛋白zeste基因增强子同源物2（EZH2）的表达，分析DNA甲基化与MM的关系。方法选取MMU266细胞株及10例MM患者BMMNC（MM—BMMNC），同时选取10名健康人骨髓提取的单个核细胞（N—BMMNC）。用Westernblot法测定细胞EZH2的含量，以目的蛋白EZH2与内参照8一actin表达量的比值作为EZH2蛋白相对表达水平。结果与10例N—BMMNC（0．2277±0．1306）比较，EZH2在U266细胞（0．9730±0．1029）和10例MM—BMMNC（0．9220±0．1301）中表达明显升高，差异有统计学意义（P〈0．05）。结论EZH2在MMU266细胞株及MM—BMMNC中含量明显增多，EZH2与MM的发生和发展机制可能有密切联系。
Objective To investigate the expression of DNA methylation related protein enhancer of zeste homolog 2 （EZH2） in multiple myeloma （MM） cell line U266 and enriched MM cells （MM-BMMNC） from 10 patients, and analyze the potential role of DNA methylation in the occurrence and development of MM. Methods Human MM cell line U266 and MM cells from l0 MM patients were studied. Enriched bone marrow mononuclear cells （N-BMMNC） from 10 healthy persons were used as the control. The expression of EZH2 were determined by Western blot using the ratio of target protein EZH2 and the internal reference β-actin expression as the final results. Results The preliminary in vitro results showed that EZH2 expression was higher in MM cell line U266 and patient MM-BMMNC （N-BMMNC 0.2277±0.1306, MM-BMMNC 0.9220±0.1301, U266 0.9730±0.1029）, with statistically significant differences （P 〈 0.05）. Conclusion EZH2 is enriched in U266 and MM-BMMNC. DNA methylation may have a close correlation with the occurrence and development of MM.
出处
《白血病．淋巴瘤》
CAS
2013年第5期284-285,293,共3页
Journal of Leukemia & Lymphoma
基金
山西省留学人员科研资助项目（200810-99）
