摘要
目的:探讨Twist基因与p53蛋白在子宫颈鳞状细胞癌(cervical squamous cell carcinoma,SCC)及子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)中的表达及其在癌变中的作用。方法:收集宫颈SCC组40例,CIN组85例(CIN Ⅰ级30例,Ⅱ级26例,Ⅲ级29例),正常宫颈鳞状上皮(正常对照组)26例。用自制组织芯片技术及免疫组织化学SP法检测Twist与p53在SCC组、CIN Ⅲ组、CIN Ⅱ组、CIN Ⅰ组中的表达状况,并与正常对照组比较。结果:正常对照组、CIN Ⅰ组、CIN Ⅱ组、CIN Ⅲ组及SCC组的Twist表达率分别为0,10.0%,42.3%,48.3%和52.5%;p53表达率分别为0,10.0%,46.2%,51.7%和52.5%;正常对照组Twist和p53的表达与CIN Ⅰ组、CIN Ⅱ组、CIN Ⅲ组及SCC组比较,差异均有统计学意义(均P<0.05)。SCC组Twist与p53呈正相关(r=0.683,P<0.05)。结论:Twist和p53随着CIN从轻到重至SCC,表达逐渐增强,表明两者共同参与了SCC癌变的发生、发展过程。联合检测Twist和p53对CIN分级、宫颈SCC的病理诊断具有重要意义。
Objective： To examine the expression of Twist and p53 in cervical intra-epithelial neoplasia （CIN） and cervical invasive squamous cell carcinoma （SCC）, and to explore the significance of their expression in the carcinogenesis of cervix. Methods： Twist and p53 expression in samples from 85 patients with CIN （including CIN I group, CIN II group, CIN Ⅲgroup）, 40 patients with cervical invasive SCC （SCC group） and 26 cases of normal cervical epithelia （control group） were examined by home-made tissue chip and immunohistochemical staining （SP）. Results： a-he positive rate for Twist expression in the control group, the CIN I group, the CIN II group, the CINIII group and the SCC group was 0, 10.0%, 42.3%, 48.3% and 53.5%, respectively; for p53 expression was 0, 10.0%, 46.2%, 51.7% and 52.5%, respectively, q-he expressions of Twist and p53 in the SCC group and the CIN groups were significantly higher than those in the control group （P〈0.05）. Twist expression was positively correlated with p53 expression in the SCC group （r=0.683, P〈0.05）. Conclusion： Overexpression of Twist and p53 may play an important role in the carcinogenesis of cervix development, and the detection of Twist combined with p53 may be helpful for the diagnosis of CIN and cervical SCC.
出处
《国际病理科学与临床杂志》
CAS
2013年第2期105-111,共7页
Journal of International Pathology and Clinical Medicine
