摘要
建立酶标仪性能评价和质量控制的方法.方法 配制系列甲基橙溶液,用参考分光光度计对酶标仪进行评价和质控,评价指标为重复性、准确性和线性,并用参考酶标仪与此进行对比测定,比较结果符合率.结果 Spectra Ⅲ型酶标仪重复性误差＜1．0%；吸光度为0．000～2．254Abs时,准确性误差＜1．0%；有效性线性范围为0．000～2．254Abs,直线回归相关系数为0．9998,回归方程为：Y＝0．0085＋29．88X,线性误差＜1%,与参考酶标仪对比,测定符合率为99%.结论 该评价方法操作简单、结果稳定、有明确的质量判断标准,可以在各级试验室使用.
Objective The procedure was established for function evaluates and quality control on the ELISA reader． Mehtods A series of methy1 orange solution was prepared and a reference spectrophotometer was used to check the repeatability the accuracy and the linearity of the instrument． A reference ELISA reader also was used to compare the test results． Results Spectra Ⅲ instrument was checked． The repeatability is better than 1．0%． The inaccuracy is not greater than 1%at 492nm0．000～2．254Abs． the linear range is between 0．000～2．254Absr＝0．9998 Y＝0．0085＋29．88X． The linearity inaccuracy is not greater than 1%． The coincidence rate of results tested by two kind of ELISA reader was 99%． Conclusion The procedure is simple． The result is stable． The determine standard is clear． The method can be used widely．
出处
《江西医学检验》
2000年第3期147-149,共3页
Jiangxi Journal of Medical Laboratory Sciences
