摘要
目的:评价红景天苷类似物对低糖低血清培养基诱导PC12细胞损伤的保护作用。方法 :体外培养PC12细胞,采用低糖低血清培养基制备损伤模型。给予60μmol/L、300μmol/L、1 000μmol/L不同浓度类似物预保护24 h。经低糖低血清损伤24 h后,观察其对细胞形态和活力的影响。结果:部分红景天苷类似物能拮抗低糖低血清培养基损伤PC12细胞活力下降。结论:部分红景天苷类似物对低糖低血清诱导PC12细胞损伤具有保护作用。
Objective： To observe the protective effects of salidroside analogues on hypoglycemia and serum limita- tion-induced injury in PC12 cells. Method： PC12 cells were cultured under hypoglycemia and serum limitation environ- ment to establish a hypoglycemia and serum limitation model in PC12 cell line. Cultured PCI2 cells were pre-incubated with different concentrations（60 μmol/L, 300 μmol/L and 1000 μmol/L） of salidroside analogues for 24h, after which hypo- glycemia and serum limitation was applied to the cells for 24h, and the effects of them on hypoglycemia and serum limita- tion induced injury were observed. Results： MTT assay indicated that some analogues could antogonize the decrease in the cell viability. Conclusion： Part of salidroside analogues display protective effects on hypoglycemia and serum limitation-in- duced injury in PC12 cells.
出处
《交通医学》
2013年第2期118-120,124,共4页
Medical Journal of Communications
基金
国家自然科学基金资助项目(21242005)
南通市自然科学基金资助项目(AS2011016)
