摘要
以春季盆栽的‘吴屯杨’与‘荷兰杨’为试验材料,采用微透析技术,结合高效液相色谱测定不同浓度NaCl(0、200、300mmol/L)胁迫下2种杨树嫩茎质外体的内源激素变化.结果表明:盐胁迫下2种杨树嫩茎质外体内脱落酸(ABA)含量升高,其他激素含量降低;高浓度盐胁迫下脱落酸(ABA)的积累量大于中浓度盐胁迫;同等盐胁迫条件下,‘荷兰杨’需要积累较‘吴屯杨’更多的脱落酸(ABA)来抵御胁迫环境;‘吴屯杨’的抗盐平衡系数小于‘荷兰杨’;2种杨树的细胞分裂素(CTK)与吲哚乙酸(IAA),细胞分裂素(CTK)与赤霉素(GA),吲哚乙酸(IAA)与赤霉素(GA)显著相关;‘吴屯杨’的耐盐性强于‘荷兰杨’.
Taking potted ＇Populus wutunensis＇ and Populus ×euramericana cv.＇N3930＇ as experimental materials,the changes of endogenous hormone in apoplast of poplar immature stem were determined by microdialysis technique and high performance liquid chromatography,under NaCl（0,200,300 mmol/L） stress.The results showed that under the NaCl stress,ABA content in two poplar apoplastic increased,while other hormones decreased.ABA accumulation under high concentration salt stress was greater than that under middle concentration salt stress.When under the same salt stress,Populus ×euramericana cv.＇N3930＇ accumulated more ABA than ＇Populus wutunensis＇ to resist the coercive environment;salt balance coefficient of ＇Populus wutunensis＇ was less than that of Populus ×euramericana cv.＇N3930＇.CTK and IAA,CTK and GA,IAA and GA of the two poplar were significantly correlated.The results indicated that salt tolerance of ＇Populus wutunensis＇ was stronger than that of Populus ×euramericana cv.＇N3930＇.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2013年第2期62-66,73,共6页
Journal of Gansu Agricultural University
基金
国家自然科学基金项目(30872004)
辽宁省重点农业攻关计划项目(2008207001)
中央高校基本科研业务费资助项目(DC12010204)(DC12010305)
关键词
杨树
微透析
内源激素
盐胁迫
质外体
poplar
microdialysis
endogenous hormones
salt stress
apoplast
