摘要
以‘青岛百合’为试验材料,研究组培微环境中NAA、蔗糖、活性炭、矮壮素及光照/黑暗条件等因素对‘青岛百合’生根的影响.结果表明:蔗糖对‘青岛百合’鳞茎的膨大影响较大,添加60g/L蔗糖溶液时,鳞茎长势最好;黑暗处理或添加3g/L活性炭可创造根系生长的最佳黑暗微环境,有助于促进根原基的分化,能提前生根2～3d;2g/L矮壮素有助于鳞茎干物质含量的增加累积,提高增强组培苗抗性;1/2MS+0.1mg/L NAA+60g/L蔗糖溶液+3g/L活性炭或进行暗处理可在短时间内获得大量组培苗;1/2 MS+0.1mg/L NAA+60g/L蔗糖+2g/L矮壮素+光照培养可获得健壮的组培苗.
To obtain the optimum culture media for bulblets rooting of Lilium tsingtauense,the influences of the concentration of NAA,sucrose,activated charcoal and CCC on the rooting of Lilium tsingtauense in vitro microenvironment were studied.The results indicated that the sucrose could significantly influence the enlargement of blublets,the plantlets grow well when 60 g/L sucrose was added in the media;dark treatment or 3 g/L activated charcoal could build dark microenvironment for root growth,which was beneficial to promote the differentiation of root primordial so that the bulblets rooted faster,under which the roots emerged 2~3 d earlier than other treatments;2 g/L CCC could increase the dry weight,which enhanced the resistance of vitro shoots.It could obtain a number of vitro shoots quickly when the treatment was 1/2 MA＋0.1 mg/L Naa＋60 g/L sucrose＋3 g/L activated charcoal or dark treatment;it could obtain strong vitro shoots when the treatment was 1/2 MS＋0.1 mg/L NAA＋60 g/L sucrose＋2 g/L CCC as well as under illumination condition.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2013年第2期40-45,共6页
Journal of Gansu Agricultural University
基金
‘青岛百合’新品种培育项目(09-3-3-17-NSH)
关键词
青岛百合
微环境
生根
Lilium tsingtauense
microenvironment
rooting
