摘要
为获得龙牙百合高品质的鳞球种球,加快组培快繁进程,以龙牙百合小鳞芽为试材,通过组织培养的方法进行了鳞球增重及生根培养研究.结果表明,诱导龙牙百合小鳞芽生长发育成鳞球的最适培养基为MS+IAA1.0mg/L;龙牙百合鳞球增重的最佳培养基为MS+6-BA1.0mg/L+2,4-D0.5mg/L,培养基蔗糖质量分数影响鳞球的增重,适当提高蔗糖质量分数对鳞球的增重有促进作用,以5%效果最好;鳞球生根的最适培养基为1/2MS+IBA0.5mg/L.
Using buds in Lilium brownii var. viridulum as research material, by the way of tissue culture, we have drawn the following conclusions： The experiments showed that the medium MS supplemented with 1.0 mg/L IAA was fitting for inducing buds change to bulbs. The medium MS supplemented with 6-BA1.0 mg/L and 2,4-D0.5mg/L was fitting for putting on weight, and the density of bagasse affected putting on weight, using 5 percent bagasse will get better result. The medium 1/2MS supplemented with IBA 0.5 mg/L was perfect for growing roots.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第4期375-377,共3页
Journal of Hunan Agricultural University(Natural Sciences)
基金
长沙市科技计划重大专项(K051003-22)
关键词
龙牙百合
鳞球增重
生根
Lilium brownii var. viridulum
bulbs putting on weight
growing roots
