摘要
溶血磷脂酸酰基转移酶在植物油脂合成途径中具有重要的作用。本研究通过构建花生种子全长cDNA文库,结合大规模EST测序和RACE克隆等方法,从花生中克隆得到了三个LPAT基因,分别命名为AhLPAT2,AhLPAT4和AhLPAT5。其中AhLPAT2全长为1626bp,ORF为1164bp,理论分子量为43.2kD,理论等电点为9.42;AhLPAT4全长为1618bp,ORF为1152bp,理论分子量为43.9kD,理论等电点为9.23;AhLPAT5全长为1911bp,ORF为1137bp,理论分子量为43.7kD,理论等电点为8.99。它们推导的氨基酸序列与拟南芥的同源性依次为78%,65%和65%;与大豆的同源性分别是82%,80%和82%。将这三个基因在GenBank上注册,注册号分别为JN032677,KC160499,KC160500。
Lysophosphatidic acid acyltransferase （LPAT） is a key enzyme in biosynthesis pathway of vegetable oil in plant. We constructed a full-length cDNA library of peanut （Arachis hypogaea） seed via a large number of sequences of expressed sequence tag （EST） and RACE clone, three lyso- phosphatidic acid acyltransferase genes, designated AhLPAT2, AhLPAT4 and AhLPATS, were iso- lated from peanut. The sequence of AhLPAT2 cDNA was 1626 bp, and ORF was 1164 bp. The mo- lecular weight of AhLPAT2 was 43.2 kD and its isoelectric point （pI） was 9.42. The sequence of AhLPAT4 and AhLPAT5 cDNA were 1618 bp and 1911 bp, and ORF were 1152 bp and 1137 bp, re- spectively. The molecular weight of AhLPAT4 and AhLPAT5 were 43.9 kD and 43.7 kD, and isoe- lectric points （pI） were 9.23 and 8.99, respectively. The deduced amino acid had a high identity withthe LPAT proteins reported from other species. Amino acid similarities of AhLPAT2, AhLPAT4 and AhLPAT5 between peanut and Arabidopsis thaliana were 78%, 65 % and 65 %, respectively; and a- mino acid similarities of AhLPAT2, AhLPAT4 and AhLPAT5 protein between peanut and Glycine max were 82%, 80% and 82%, respectively. The sequences of the three genes were also submitted to GenBank and their GenBank ID were JN032677, KC160499, KC160500, respectively.
出处
《花生学报》
2013年第1期1-11,共11页
Journal of Peanut Science
基金
国家产业体系(CARS-14)
山东省自然基金(ZR2009DQ004
ZR2011CQ036
ZR2012CQ031)
国家自然基金(31000728
31100205
31200211)
山东省优秀中青年科学家科研奖励基金(BS2010NY023)
青岛市科技计划(11-2-4-9-(3)-jch
11-2-3-26-nsh
12-1-4-11-(2)-jch)
关键词
LPAT
花生
基因克隆
序列分析
lysophosphatidic acid acyltransferase
peanut （Arachis hypogaea L. ）
gene cloning
sequence analysis
