摘要
目的:通过分析肾素-血管紧张素系统(RAS)3个关键基因血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态、血管紧张素Ⅰ转化酶(ACE)基因插入/缺失(I/D)多态和血管紧张素原(AGT)基因M235T多态在壮族IgA肾病患者中的分布,进而探讨RAS基因多态在壮族IgA肾病预后判断中的价值。方法:选取壮族IgA肾病患者68例(肾病组),并选取70例健康体检者作为健康对照组。采用直接聚合酶链反应和聚合酶链反应-限制性片段长度多态性技术检测RAS ACE基因I/D多态、AT1R基因A1166C多态和AGT基因M235T多态,并分析其与肾脏病变程度、蛋白尿、高血压、肾功能损害等的关系。结果:肾病组ACE基因I/D多态性与健康对照组比较差异有统计学意义,DD基因型和D等位基因在肾病组中占显著优势(P<0.05),而不同HAAS病理分级比较,ACE I/D多态DD基因型在≥Ⅲ级组中占显著优势(P<0.05),肾损害组DD基因型分布频率高于无肾损害组(P<0.05),ACE基因I/D多态在伴蛋白尿组和不伴蛋白尿组、伴高血压组和不伴高血压组中的分布差异均无统计学意义;各组中AT1R A1166C和AGT M235T基因型与等位基因分布频率差异均无统计学意义。结论:ACE I/D多态的DD基因和D等位基因是壮族IgA肾病发生的易感因素之一,携带DD基因患者易于表现为严重病理分级和出现肾功能损害,DD基因型是预测壮族IgA肾病预后不良的标志。
Objective：To explore the value of renin-angiotensin system（RAS）gene polymorphism analysis in prognosis of IgA nephropathy in Chuang population.The distributions of angiotensin Ⅱ type 1 receptor（AT1R） gene A1166C polymorphism,angiotensin I converting enzyme（ACE） gene insertion/deletion（I/D） polymorphism and angiotensinogen（AGT） gene M235T polymorphism in Chuang patients with IgA nephropathy were studied.Methods：68 cases of Chuang patients with IgA nephropathy（nephropathy group） were selected.And then 70 cases of people as healthy control group were selected.RAS ACE gene I/D polymorphism,AT1R gene A1166C polymorphism and AGT gene M235T polymorphism in each group were detected by direct polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism technique.The association of 3 genes polymorphism with renal pathological degree,proteinuria,hypertension and renal damage were analyzed.Results：The difference of ACE gene I/D polymorphism in nephropathy group compared with that in health control group were statistically significant,the distribution of DD genotype and D allele in nephropathy group accounted for a significant advantage.The distribution of D allele in ≥ Ⅲ grade group accounted for a significant advantage.The frequency distribution of DD genotype in renal damage group was higher than that in without renal damage group（P〈0.05）.The distribution of ACE gene I/D polymorphism in proteinuria group and without proteinuria group,hypertension group and without hypertension group were no significant differences.While genotype and allele frequency distributions of AT1R A1166C and AGT M235T had no significant difference between groups.Conclusion：DD gene and D allele of ACE I/D polymorphism are the predisposing factors of IgA nephropathy.Patients carrying the DD gene show severe pathological grade and impairment of renal function.DD genotype is a predictive marker of poor prognosis of IgA nephropathy in Chuang population.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第5期503-507,共5页
Chinese Journal of Immunology
基金
广西自然科学基金资助项目(No.桂科青0542074)
广西教育厅科研资助项目(No.201012MS180)
