摘要
目的研究侵袭性垂体腺瘤病理形态学变化特征及细胞核内增殖抗原阳-67、基质金属蛋白酶-9（MMP-9）在侵袭性、非侵袭性腺瘤中的表达，探讨细胞形态变化与蛋白表达相关性及与肿瘤侵袭性的影响。方法分析2003年1月至2011年12月手术治疗的垂体腺瘤患者手术标本125例，依据是否有侵袭性分为两组，通过光镜、电镜观察病理形态学变化特征及免疫组化二步法检测陷-67、MMP-9蛋白表达。结果侵袭性组瘤细胞高核质比、多细胞性、核异型性、出现核仁等病理形态学特征发生率明显高于非侵袭性组，两组比较差异有统计学意义（P〈0．05）；电镜在侵袭性组观察到核异型及核仁边集现象。同时发现侵袭性组Ki-67、MMP-9蛋白表达明显高于非侵袭性组（P〈0．01），差异有统计学意义；且细胞形态变化与Ki-67、MMP-9蛋白表达变化呈正相关。结论垂体腺瘤Ki-67、MMP-9蛋白表达增高与肿瘤侵袭性存在相关性，细胞病理形态学变化对肿瘤侵袭性的诊断具有重要的参考价值。
Objective To study the pathological morphologic characteristics of invasive pituitary tumors and the affect of expression of Ki -67 and MMP -9 to the tumor invasion. Methods Analyzing 130 cases of pituitary tumor specimens from January 2003 to december 2011, which divided into two groups according to invasion, observe pathological morphologic characteristics through the light and electron microscope and the expression of Ki -67 and MMP -9 via immunohistochemistry （IHC）. Results The difference was statistically significant（ P 〈0.05 ） between the invasive group and noninvasive group in the incidence rate of pathological morphologic characteristics, such as high nuclearcytoplasmic ratio, cell pleomorphism, nuclear atypia, and nucleoli appearenee; nuclear atypia and nucleolus margination in the invasive group were found through the electron microscopy, therein addition, significant statistical differences in the rate of Ki - 67 and MMP - 9 expressions were found ; the rate was higher in the invasive group than the noninvasive group（ P 〈 0.01 ）. Conclusions the pathological morphologic characteristics of pituitary tumor and the high expression of Ki - 67 and MMP - 9 were significantly valuable in the diagnosis of tumor aggressiveness, which provided valuable indicators for early clinical diagnosis of tumor invasion.
出处
《中华神经外科杂志》
CSCD
北大核心
2013年第5期465-468,共4页
Chinese Journal of Neurosurgery
基金
江苏省科委基金项目（jg1006苏科计143号文）
