摘要
目的:观察外源性H2S供体NaHS对海洛因依赖大鼠学习记忆能力及海马LTP的影响。方法:SD大鼠随机分成3组:正常对照组、heroin组、heroin+NaHS组。先通过跳台实验检测大鼠学习记忆能力,然后记录高频刺激(HFS)前后在体海马CA1区群体峰电位(PS)变化,诱导长时程增强(LTP)的产生,最后通过Nissl染色观察海马神经元的损伤情况。结果:与对照组比较,heroin组和heroin+NaHS组学习记忆成绩均降低(P<0.05),PS幅值变化率减小(P<0.01),且形态学观察可见海马神经元损伤;与heroin组比较,heroin+NaHS组学习记忆成绩提高(P<0.05),PS幅值变化率增大(P<0.01),海马神经元损伤较轻。结论:(1)海洛因依赖导致大鼠海马神经元损伤,抑制海马CA1区LTP的产生,从而降低正常学习记忆能力;(2)外源性H2S可减轻海洛因依赖对大鼠海马神经元的损伤,易化海马CA1区LTP的产生,改善海洛因依赖导致的正常学习记忆能力的降低。
Objective： To observe the effects of exogenous hydrogen sulfide on learning and memory, hippocampal longterm potentiation in rats with heroin dependence. Methods： Sprague Dawley rats were randomly divided into three groups ： control group, heroin group and heroin ＋ Naris group. Learning and memory ability was examined by step-down test. The change of the amplitude of population spike （PS） and long-term potentiation （LTP） induction were recorded in hippocampal CA1 area before and after high frequency stimulation （HFS）. The damage of hippocampal neuron was observed by Nissl staining. Results ： Compared with control group, learning and memory ability reduced （ P 〈 0.05 ）, the change of the amplitude of PS in hippocampal CA1 area was decreased （ P 〈 0.01 ）, the damage of hippocampal neuron was observed in heroin group and heroin ＋ Naris group. Compared with heroin group, learning and memory ability raised （P 〈 0.05 ） , the change of the amplitude of PS in hippocampal CA1 area was increased （P 〈 0.01 ）, the damage of hippocampal neurons was alleviated in heroin ＋ Naris group. Conclusions ： （ 1 ） Heroin dependence resulted in the damage of hippocampal neurons, inhibited LTP induction in hippocampal CA1 area, and consequently reduced the ability of learning and memory in rats ; （2） Exogenous H2 S reduced the damage of hippocampal neurons, facilitated LTP induction in hippocampus CA1 area, and improved learning and memory ability in heroin dependence rats.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2013年第3期316-320,共5页
Chinese Journal of Neuroanatomy
基金
贵州省高层次人才科研条件特助经费项目(TZJF-2008-59)
关键词
硫化氢
海洛因依赖
海马
长时程增强
学习记忆
大鼠
hydrogen sulfide
heroin dependence
hippocampus
long-term potentiation
learning memory
rat
