摘要
目的:观察卡维地洛对病毒性心肌炎小鼠的治疗疗效和对白细胞介素(白介素)-8因子表达水平的影响,探讨其抑制心肌炎症反应的机制。方法:将75只SPF级近交系4-6周雄性BALB/c小鼠随机分成4组,空白对照组15只、病毒组无干预组20只、卡维地洛干预组20只、维生素C组干预组20只,观察各组小鼠10 d后生存率,对存活小鼠眼球取血后处死,留取心脏及血液标本。光镜观察小鼠心肌组织病理改变情况,ELISA法检测血液中白介素-8及Western blot检测心肌组织中白介素-8表达水平。结果:卡维地洛组、维生素C干预后,小鼠生存率增高,光镜下小鼠心肌组织的炎症细胞浸润较病毒组减轻,白介素-8因子表达水平低于病毒组,卡维地洛组干预组指标下降更加显著。结论:卡维地洛和维生素C可以通过抑制白介素8的合成,减轻病毒性心肌炎的炎症反应,从而保护心肌细胞,卡维地洛疗效更佳。
Objective： To observe the curative effect of carvedilol and the expression of interleukin（IL）-8 in a murine model of viral myocarditis（VMC）.Methods： A total of 75 inbred male BALB/c mice of 4-6 weeks old were divided at random into four groups as follows： blank group（n = 15）,VMC control group（n =20）,carvedilol-administered group（n = 20）,and vitamin C-administered group（n = 20）.Two weeks later,the mice were sacrificed after observing the survival rate and gathering blood specimens and myocardial tissue.The histological changes were observed under light microscope.The serum IL-8 concentrations were detected by ELISA.Western blot was performed to analyze the contents of IL-8 in myocardium.Results： Compared with VMC control group,carvedilol and vitamin C improved survival rate, and reduced the number of inflammatory cell infiltrating in myocardium at acute stage.The IL-8 expression significantly decreased in carvedilol and vitamin C groups,and it decreased more significantly in carvedilol group than in vitamin C group.Conclusion： Both carvedilol and vitamin C,especially the former,could protect against VMC by reducing inflammatory reaction.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2013年第3期360-363,共4页
Medical Journal of Wuhan University
