摘要
对虾WSSV病是亚洲对虾养殖业中的一个棘手问题。本研究采用Kimura引物 ,用PCR技术对不同生长期的中国对虾 (Penaeuschinensis)进行了WSSV的检测 ,同时也检测了对虾发病时养殖池中多见的野生厚蟹 (Helicesp .)和矛尾刺虎鱼 (Acanthogobiushasta)。检测结果表明 :分别在检测的 5尾亲虾中的 1尾 ,6尾仔虾中的 1尾 ,5尾稚虾中的 3尾及所检测的 5尾病虾和 2只厚蟹中获得到 982bp的PCR扩增产物 ,说明为WSSV感染阳性。在检测的 2尾矛尾刺虎鱼中均未获得PCR扩增产物 ,说明为WSSV感染阴性。在亲虾、虾苗以及虾池内的野生厚蟹中检测到WSSV感染的阳性结果表明 :WSSV感染的亲虾有可能是病毒的储主 ,WSSV感染的野生厚蟹有可能是病毒中间宿主或病毒的携带者 ,它们在对虾WSSV病的感染。
White spot syndrome virus (WSSV) disease is a key problem in shrimp culture industry in Asian countries. The present study attempted to detect the WSSV infected in some developmental stage of Penaeus chinensis by PCR using Kimura primer. And wild crab (Helice sp.) and javelin goby (Acanthogobius hasta) captured from shrimp culture pond were also detected. The detecting results showed that one out of five shrimp spawners, one out of six postlarvae, three out of five juveniles, all of five diseased shrimps and all of two crabs yielded a 982 bp PCR product while two javelin goby no PCR product appeared. WSSV positive was obtained from the spawners, seedings and wild crabs in shrimp culture ponds. It was indicated that WSSV-infected spawners maybe the virus reservoir and the wild crabs maybe the virus inter-host or carrier probably. They play an important role in transmission and spreading of shrimp WSSV disease.
出处
《中国水产科学》
CAS
CSCD
2000年第1期51-54,共4页
Journal of Fishery Sciences of China
基金
8 63海洋领域海洋生物技术资助项目! (819-Q - 0 8)
国家重点基础研究规划项目! (G19990 12 0 0 2 )
教育部留学回国人员科研启动
