摘要
用 2 4种随机引物对雅罗鱼亚科 (Leuciscinae)的草鱼、鲤亚科 (Cyprininae)的柏氏鲤和 3个地理种群鲤 (荷包红鲤、黑龙江野鲤和德国镜鲤 )进行RAPD分析 ,构建了这 5种鱼类的基因组指纹图谱。通过对获得的基因组指纹图谱的量化分析 ,利用UPGMA重建了它们之间的亲缘关系。结果鲤种内 3个地理种群之间的相似性高于鲤和柏氏鲤种间的相似性 ,而草鱼与另 4种鲤亚科鱼类亚科之间的差异明显高于柏氏鲤、黑龙江野鲤、荷包红鲤和德国镜鲤的种间或种内差异。
Genomic fingerprints of 5 species/strains: grass carp(subfamily Leuciscinae)、Cyprinus pellegrini Tchang(subfamily Cyprinidae)and 3 geographic groups of Cyprinus carpio haematopterus (purse red carp、common carp and mirror carp, subfamily Cyprinidae), were constructed with 24 RAPD primers. By analysing the data of genomic fingerprints,the genetic relationship of the 5 species/strains was reconstructed with UPGMA. The reconstructed phylogeny showed that the relationships between the 3 geographic groups of Cyprinus carpio haematopterus were more closer than that of the 3 with Cyprinus pellegrini Tchang, and the distances between grass carp and the 4 species groups of subfamily Cyprinidae was more obvious than those within subfamily. The result supports the opinion that the fish in east China have two-fold origination.
出处
《中国水产科学》
CAS
CSCD
2000年第1期6-11,共6页
Journal of Fishery Sciences of China
基金
国家高技术研究发展计划资助项目! (863 - 10 1- 0 5 -0 1)
关键词
RAPD指纹图谱
草鱼
柏氏鲤
亲缘关系
RAPD fingerprints
grass carp
Boshi carp
purse red carp
common carp
mirror carp
phylogeny family
