摘要
目的：研究黄曲霉毒素高危区谷胱甘肽转移酶M1(GSTM1)基因多态性与肝细胞癌的相关性，验证该指标作为肝细胞癌预报因子的可靠性。方法：用特异性引物与PCR技术，检测不同地区正常人、高污染区肝细胞癌患者的GSTM1缺失的基因型频率。结果：黄曲霉毒素高危区肝细胞癌患者与正常人的GSTM1基因缺失频率分别为59％和52％，无显著性差异；但与肝细胞癌低发地区正常人相比，其基因缺失频率较高，差异具有显著性意义(P<0.01)。结论：在黄曲霉毒素污染严重地区，单一解毒酶GSTM1的作用难以对抗过量黄典霉毒素B1(AFB1)的致癌活性，其基因缺失不能作为预报肝细胞癌易罹性的独立指标，在筛选易罹者时，必须综合考虑环境致癌物污染浓度、HBV感染及肝细胞损伤程度和多种遗传因素的共同作用。
Objective： To investigate the relationship between glutathione S transferase M1 gene polymorphisms and hepatocellular carcinoma(HCC) and the reliability of GSTM1 gene deletion as a predictor of HCC. Methods： Both the GSTM1 gene deletion of peripheral white blood cells of 100 normal subjects and 120 HCC patients from the same high AFB1 contaminated area were detected by using PCR technique with special primer. Another 40 normal cases from AFB1 low risk area were also tested. Results： In HCC high risk area, the frequencies of GSTM1 null genotype in normal subjects and the patients were 52％ and 59％ , respectirey, which was not significantly different. Howerer, the frequency of GSTM1 null genotype in control from HCC low risk area was lower than these of from high risk area (P< 0.01). Conclusions： The detoxification effect of GSTM1 alone may not be able to resist the carcinogenesis of AFB1, expecially in those people who over exposed to AFB1. The susceptibility to HCC may not be predicted by GSTM1 deletion. The synergistic effects among HCC risk factors such as HBV, chronic hepatitis and many genetic variations must be considered when identify any susceptible individual.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第9期868-870,共3页
Chinese Journal of Cancer
基金
本课题获国家自然科学基金!(No39860032)
广西教育厅重点资助课题基金!(No98-2-8)资助
