摘要
目的：研究广西不同地区肝细胞癌（ＨＣＣ）ｐ５３基因２４９密码子（ｃｄ２４９）的突变热点与黄曲霉毒素Ｂ１（ＡＦＢ１）污染及ＨＢＶ感染的关系。方法：用ＰＣＲ－ＲＦＬＰ法检测６２例石蜡包埋ＨＣＣ组织中上述基因突变，免疫组化染色Ｓ－Ｐ法检测癌旁肝组织ＨＢｓＡｇ表达。结果：广西ＨＣＣ高发区及低发区病例ｐ５３基因ｃｄ２４９的突变频率分别为６９．２％及２０％（Ｐ＜０．０１）。癌旁肝组织ＨＢｓＡｇ的阳性率为８１％及１００％（Ｐ＞０．０５）。ｐ５３基因突变热点与ＡＦＢ１严重污染及ＨＣＣ高发有密切关系，而与ＨＢＶ感染无关。结论：ｐ５３基因突变热点的出现具有分子流行病学意义。ｐ５３热点高频率出现表明了当地ＡＦＢ１的严重污染及ＨＣＣ发生的主要地区性因素。
Purpose To study the frequency of p53 gene mutational hotspot and its relation to HBV in two groups of hepatocellular carcinoma cases. Methods The paraffinembedded tissues of 62 cases of HCC were studied by polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP) analysis and immunohistochemical SP staining. Results The frequencies of the p53 mutational hotspot were 692% and 20% respectively in the two groups of HCC cases which came separately from high and low Aflatoxin B1 risk area (P<001). The mutational hotspot was not related to HBV. Conclusions The mutational hotspot of p53 gene of HCC is of molecular epidemiological significance, which indicates the serious contamination of AFB1 in foods and the main etiological factor of HCC in the area.
出处
《临床与实验病理学杂志》
CAS
CSCD
1997年第4期302-304,共3页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金
