摘要
目的:探讨雷公藤甲素对大鼠C6胶质瘤细胞的增殖诱导作用及其相关机制。方法:将大鼠C6胶质瘤细胞培养于含10%(FBS),100 mg.L-1青霉素链霉素的DMEM培养液中,置37℃5%CO2培养箱中培养,采用四甲基偶氮唑盐比色(MTT)法检测雷公藤甲素对大鼠C6胶质瘤细胞的抑制作用,流式细胞仪(FCM)检测C6胶质瘤细胞凋亡情况,用逆转录-聚合酶链反应(Real Time-Polymerase Chain Reaction,RT-PCR),免疫印迹法(Western blotting,WB)检测细胞淋巴瘤/白血病-2(Bcl-2),Bcl-2相关X蛋白(Bax)mRNA及蛋白的表达变化情况。结果:1.2 mg.L-1浓度的雷公藤甲素溶液对C6胶质瘤细胞增殖有抑制作用,且呈明显的剂量依赖性;雷公藤甲素作用C6胶质瘤细胞24 h后,1.2 mg.L-1浓度对其凋亡诱导作用最为明显;雷公藤甲素可明显下调细胞中Bcl-2 mRNA及蛋白质表达,且该作用呈剂量-时间依赖性,而Bax mRNA及蛋白质表达呈上调趋势。结论:雷公藤甲素对C6胶质瘤细胞具有较好的生长抑制和凋亡诱导作用,而其作用机制可能与Bcl-2,Bax mRNA及蛋白质表达有关。
Objective： To investigate the effect of triptolide （TPL） on proliferation and apoptosis of C6 cells. Method： C6 cells was cultured with different concentrations of TPL for different hours. MTT assay was employed to evaluate the survival of TPL-treated C6 cells. The induction of apoptosis was detected by flow cytometry analysis （FCM）. The expressions of B cell lymphoma/lewkmia-2（Bcl-2） and Bcl-2 assaciated x protein（Bax） mRNA were detected by Real Time - Polymerase Chain Reaction （RT-PCR）, while the protein by Western blotting （WB） methods. Result： Our results showed that TPL induced apoptotic cell death in a dose- and time-dependent manner, which was accompanied by the regulation of Bcl-2 and Bax expressions. Conclusion： The results indicated that TPL induce the apoptosis and inhibit the proliferation of C6 glioma cellsby regulating the expressions of Bcl-2 and Bax.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期246-249,共4页
Chinese Journal of Experimental Traditional Medical Formulae
