摘要
目的:探讨左归丸对庆大霉素所致肾小管损伤大鼠c-Jun氨基末端激酶(JNK)的影响。方法:将40只雄性Wistar大鼠随机分为空白对照组(C组),模型组(M组),c-Jun氨基末端激酶特异性抑制剂(SP600125)组(S组),左归丸组(Z组),每组10只,对照组ip给予0.9%生理盐水2.5 mL.kg-1.d-1,模型组ip硫酸庆大霉素100 mg.kg-1.d-1,SP600125组左侧ip硫酸庆大霉素100 mg.kg-1.d-1,2 h后右侧ip SP600125 15 mg.kg-1.d-1,左归丸组ip硫酸庆大霉素100 mg.kg-1.d-1和ig左归丸水溶液10 g.kg-1.d-1,4组大鼠连续给药10 d。第11日测量各组大鼠尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG);HE染色观察各组大鼠肾脏病理状况;免疫组化技术检测各组大鼠肾脏JNK,c-Jun的表达;以Western blot方法检测各组大鼠肾脏c-Jun氨基末端激酶(JNK),c-Jun蛋白的表达。结果:尿NAG酶结果显示:与C组比较,M组尿NAG酶显著升高(P<0.01);与M组比较,S组和Z组尿NAG酶下降(P<0.05)。HE染色结果显示:C组肾小管、间质无特殊改变;M组肾小管上皮细胞出现空泡变性、管腔出现损伤,有大量的炎性细胞浸润;S组与Z组肾小管少量炎性细胞浸润,轻微肿胀变性。免疫组化结果显示:与C组比较,M组JNK,c-Jun在肾小管大量表达(P<0.01);与M组比较,S组和Z组JNK,c-Jun在肾小管表达较少(P<0.01)。免疫印迹杂交结果显示:M组JNK,c-Jun蛋白灰度值较对照组明显升高(P<0.01),S组与Z组较M组灰度值下降(P<0.01或P<0.05)。结论:左归丸对庆大霉素所致肾小管损伤大鼠的保护作用可能与抑制c-Jun氨基末端激酶的激活有关。
Objective： Effect of Zuogui Wan on the expression of c-Jun amino-terminal kinase（JNK） and c-Jun in rats with gentamicin-induced renal tubular injury. Method： Forty male Wistar rats were divided randomly into control group（C）, model group（M）, Sp600125 group（S） and Zuoguiwan group（Z）. The C group was given ip injection of 0.9% saline 2.5 mL·kg-1·d-1, M group was given ip injection of gentamicin sulfate 100 mg·kg^-1·d^-1, S group was given ip injection of gentamicin sulfate 100 mg·kg^-1·d^-1 and ip injection of SP600125 15 mg·kg^-1·d^-1 Zuoguiwan group was given ip injection of gentamicin sulfate 100 mg·kg^-1·d^-1 and gavage Zuoguiwan aqueous solution 10 g·kg^-1·d^-1. Four groups of rats administered for 10 consecutive days. After the experiment measuring the NAG of rat. Hematoxylin-eosin to detect renal histopathology. Immunohistochemical techniques to detect JNK and c-Jun＇s expression of the rat kidney. Western blot techniques to detect JNK and c-Jun＇s expression of the rat kidney. Result： The results of biochemical index show： compared with the C group, the rats of M group NAG significantly increased, with a statistically significant difference （P〈0.01）; compared with the M group, the biochemical parameters improved of S group and Z group, with a statistically significant difference （P〈0.05）. The HE staining results show： the C group of renal tubule, renal interstitial no change; the M group of renal tubular epithelial cells swelling, vacuolar degeneration. The S group and Z group renal tubule epithelial cells slight swelling, degeneration. The immunohistochemical results show： compared with the C group, the rats of M group JNK, c-Jun abundantly expressed in the renal tubules, with a statistically significant difference （P〈0.01）; compared with the M group, protein expression was not significant in the S group and Z group, with a statistically significant difference （P〈0.01）. The results of western blot show： compared with the C group, the rats of M group JNK, c-Jun＇s gray values increased significantly, with a statistically significant difference （P〈0.01）; compared with the M group, the gray values of S group and Z group decreased, with a statistically significant difference （P〈0.05, P〈0.01）. Conclusion： The protective effect of Zuogui Wan relieved the gentamicin-induced renal tubular injury in rats, which mechanism may be involved in the inhibition of the c-Jun N-terminal kinase activation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期194-198,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家临床重点专科建设项目(FW20113289)
