摘要
目的评价腹腔注射不同剂量异丙酚对哮喘小鼠气道高反应性、肺部炎症以及Th1／Th2平衡的调节作用。方法健康雌性BALB／c小鼠100只，6～8周龄，体重18～20g，采用随机数字表法，将小鼠分为5组（n=20）：对照组（C组）、哮喘组（A组）、低剂量异丙酚组（50mg／kgi．P．，LP组）、中等剂量异丙酚组（100mg／kgi．p．，MP组）和高剂量异丙酚组（150mg／kgi．P．，HP组）。使用卵清蛋白（OVA）构建过敏性哮喘模型小鼠，腹腔注射＋诱导过敏性哮喘模型。各组随机取10只于模型构建成功后24h即高剂量麻醉处死，行心脏取血及支气管肺泡灌洗，以ELISA法检测血清OVA特异性IgE和支气管肺泡灌洗液中特征性细胞因子IL-4、IL-5、IFN-γ浓度，并行细胞计数／分类计数。各组剩余的10只于模型构建成功后24h行肺功能检测和取肺进行HE染色，比较各组小鼠气道反应性和肺组织的病理组织学评分。结果腹腔注射异丙酚（LP组和MP组）可明显地抑制哮喘小鼠的气道高反应性。不同剂量异丙酚干预组（LP组、MP组和HP组）均可明显抑制哮喘小鼠肺组织中嗜酸性粒细胞浸润，降低IL-4、IL-5和血清OVA特异性IgE水平，上调IFN-γ／IL-4比值。结论异丙酚能够有效抑制哮喘小鼠肺部炎症，上调Th1／Th2比值，改善肺功能，发挥气道保护作用。
Objective To investigate the immunoregulatory effects of propofol on airway hyperresponsiv- eness, airway inflammation and Th1/Th2 ratio in the asthmatic mice. Methods One hundred female BALB/c mice,aged 6-8 weeks, weighing 18-20 g, were randomly divided into 5 groups （n = 20, each）： control group （normal saline i.p., group C）, asthma group （group A）, low-dose propofol （50 mg/kg i.p. , group LP）, medi- um-dose propofol （100 mg/kg i.p., group MP） and high-dose propofol （150 mg/kg i.p., group HP）. Mice of groups A, LP, MP and HP were sensitized with ovalbumin （OVA）, mice of group C were sensitized with normal saline. 24 h after the last challenge, animals were sacrificed by lethal dose of pentobarbital sodium. Blood and bronchoalveolar lavage fluid （BALF） were collected for determination of serum OVA-specific IgE and the levels of cytokines （IL-4, IL-5 and IFN-γ） in the BALF. Airway responsiveness was measured by the forced-oscillation technique and histological inflammation scores were measured by staining with hematoxylin and eosin. Results Propofol （group LP and group MP） attenuated airway hyperresposiveness to the muscarinic agonist methacholine in OVA-induced asthma. Different doses of propofol （group LP, group MP and group HP） decreased eosinophils in- flux in lungs. In addition, propofol treatment reduced expression of IL-4, IL-5 and serum OVA-specific IgE and increased the ratio of IFN-γ/IL-4. Conclusion The study demonstrates a potential protective value of propofol inalleviating airway inflammation, up-regulating Th1/Th2 ratio and attenuating airway hyperresposiveness in the asth- matic mice.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2013年第3期334-337,共4页
Chinese Journal of Anesthesiology
基金
基金项目：国家自然科学基金（81250026）
关键词
哮喘
二异丙酚
呼吸超敏反应
炎症
Asthma
Propofol
Respiratory hypersensitivity
Inflammation
