摘要
采用高效液相色谱法（HPLC），选用TunerPackingKromasil氨基柱，以乙腈：水（80：20）为流动相，流速08ml／min，440nm下测定藏花素，结果表明：藏花素的保留时间为1219min，最小检测限为12．5ng，加样回收率为99．34％，色谱峰分离效果好，具有良好的稳定性和重视性。
The High-performance liquid chromatographic determination of crocin was described.Crocin was separated verywell on Tuner Packing Kromasil NH2 column by the use of acetonitrile:water (80:20) as mobile phase-under the flow rate of 0.8ml/min.It was detected at 440nm by UV-VIS spectrophtometer.the retention time was 12. 19min the detection limitwas 12.5ng.The average recovery rate was 99.34%,with a good stability and reproducibility,.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2000年第10期46-48,共3页
Food Science
