摘要
目的:对雌、雄皱纹盘鲍内脏脂质进行提取及测定,主要对其脂肪酸和磷脂组成进行分析比较。方法:Floch法提取雌、雄鲍鱼内脏脂质,测定脂质基本指标,游离脂肪酸、甘油三酯、磷脂、胆固醇含量;气相色谱法测定雌、雄鲍鱼内脏脂肪酸组成,并对其比较分析;固相微萃取法分别分离提取雌、雄鲍鱼内脏磷脂,高效液相色谱-蒸发光散射法测定雌、雄鲍鱼内脏磷脂组成并比较分析。结果:雌、雄皱纹盘鲍内脏脂质有较大差异,雌性鲍鱼内脏不饱和脂肪酸含量高于雄性;雌、雄鲍鱼内脏磷脂成分较复杂,但主要的磷脂组分为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸。结论:鲍鱼内脏中含有丰富的磷脂及多不饱脂肪酸,具有较好的食用和药用价值,应得到充分利用。
Objective： To describe comparative analysis of visceral fatty acids and phospholipids in male and female Haliotis discus hannai Ino.Methods： Visceral total lipids were extracted using Folch method and determined for the contents of free fatty acids,triglycerides,phospholipids and cholesterol.The fatty acid composition was analyzed by GC after derivatization with 10% H2SO4-CH3OH solution.Phospholipids were extracted using SPE method,and then analyzed by HPLC-ELSD.Results： The visceral content of unsaturated fatty acids in female Haliotis discus hannai Ino was higher than in male Haliotis discus hannai Ino.The composition of visceral phospholipids in male and female Haliotis discus hannai Ino,though complicated,mainly consisted of phosphatidylcholine（PC）,phosphatidylethanolamine（PE） and phosphatidylserine（PS）.Conclusion： The viscera of both female and male Haliotis discus hannai Ino are rich in phospholipids and polysaturated fatty acids and have high medicinal and edible values.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第10期184-186,共3页
Food Science
基金
海洋公益性行业科研专项(201105029)
关键词
雌雄鲍鱼内脏
脂肪酸
磷脂
气相色谱
高效液相色谱-蒸发光散射
viscera of Haliotis discus hannai Ino
fatty acids
phospholipids
gas chromatography（GC）
high performance liquid chromatography-evaportive light scattering detector（HPLC-ELSD）
