摘要
以鼠曲草为试验原料,采用浸提法提取鼠曲草色素,分别研究乙醇体积分数、料液比及浸提时间等因素对提取效果的影响,在单因素试验的基础上,通过L9(34)正交试验优化提取参数。同时考察温度、光照、溶液pH值及金属离子对鼠曲草色素稳定性的影响。结果表明,鼠曲草色素最优提取工艺为乙醇体积分数90%、料液比1:300(g/mL),浸提时间100min。鼠曲草色素的最大吸收波长为416nm,对光照和温度稳定性较好,在碱性条件下色素比较稳定,Cu2+、Ca2+及Zn2+对色素稳定性影响较大。
A solvent extraction technique was developed to extract pigment from Herba Gnaphaii Affinis and optimized by investigating the effects of ethanol concentration,solid-to-liquid ratio and extraction time on extraction efficiency using onefactor-at-a-time and orthogonal array design.The stability of the pigment obtained was also investigated to temperature,light,pH and metal ions.The optimal extraction conditions were found to be 90% ethanol,a solid-to-liquid ratio of 1：300（g/mL） and 100 min.The pigment obtained under these conditions had maximum absorption at 416 nm and was stable to light,temperature and alkaline condition but greatly influenced by Cu2＋,Ca2＋and Zn2＋.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第10期103-106,共4页
Food Science
基金
宁波大学SRIP项目(2011T29)
关键词
鼠曲草
色素
正交试验
稳定性
Herba Gnaphaii Affinis
pigment
orthogonal dsign
stability
