摘要
比较8种大孔树脂对蓝莓叶多酚的静态吸附与解吸效果,HPD400树脂吸附量为52.95mg/g,解吸率为95.96%,并且达到吸附平衡与解吸平衡的时间较短,最适合蓝莓叶多酚的纯化。当上样质量浓度3mg/mL、pH2、上样流速2mL/min、上样体积140mL时,树脂带到动态吸附饱和;用80mL 40%的乙醇,以1mL/min的流速进行洗脱,可以洗脱完全,解吸率为90.50%。经过HPD400树脂的纯化,蓝莓叶多酚的纯度由原来的38.75%提高到69.38%。HPLC-DAD-MS的分析结果显示,蓝莓叶多酚含有丰富的咖啡酰奎宁酸与槲皮素甙。
Adsorption and desorption of 8 types of macroporous resins towards polyphenols from blueberry leaves were investigated.The adsorption capacity and desorption rate of HPD400 resin was 52.95 mg/g and 95.96% respectively,and the time to reach equilibrium between adsorption and desorption was short,so it was the most suitable resin to purify blueberry leaves polyphenols.When 140 mL of 3 mg/mL samples with pH 2 was flowed through the HPD400 resin at a speed of 1 mL/min,the adsorption reached dynamic equilibrium.The adsorbed polyphenols could be completely eluted with 80 mL of 40% ethanol at 1 mL/min,resulting in a desorption rate of 90.50%.After being purified with HPD400 resin,the purity of polyphenols was increased from 38.75% to 69.38%.HPLC-DAD-MS analysis showed that the blueberry leaf polyphenols were rich in caffeoylquinic acids and quercetin glycosides.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第10期86-91,共6页
Food Science
基金
2011年南京市科技发展计划国际联合研发项目(201101093)
