摘要
通过静态吸附与解吸实验对4种大孔树脂进行筛选,结果表明AB-8树脂对长白楤木嫩芽皂苷的吸附和解吸效果最好。动态吸附解吸实验表明:AB-8树脂纯化长白楤木嫩芽皂苷的最佳条件为上柱液皂苷质量浓度0.2mg/mL,上柱液体积20BV,梯度洗脱实验结果表明50%和70%乙醇洗部位皂苷含量和纯度较高。对50%和70%乙醇梯度洗脱所得纯化皂苷样品进行清除超氧阴离子自由基和抑菌实验结果表明,二者均具有一定的清除超氧阴离子自由基和抑菌的活性。
Static adsorption and desorption experiments were carried out to screen an optimal one from 4 types of macroporous adsorption resins for the purification of saponin from edible stems of Aralia continentalis.AB-8-type macroporous resin was shown to be an optimum candidate for saponin purification.Subsequently,dynamic adsorption and desorption experiments were performed to optimize technological conditions for saponin purification using AB-8-type macroporous resin.Results showed that the optimal adsorption conditions were obtained as follows：saponin concentration 0.2 mg/mL and sample injection amount 20-fold bed volume （BV）.Two highly pure saponin fractions having strong superoxide anion radical scavenging capacity and antibacterial potential were obtained from gradient elution with 50% and 70% ethanol,respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第4期73-76,共4页
Food Science
基金
中国博士后科学基金资助项目(20080431155)
关键词
长白楤木
皂苷
大孔树脂
Aralia continentalis
saponin
macroporous adsorption resin
