摘要
目的观察类叶升麻苷对大鼠原代肝细胞免疫性肝损伤的保护作用。方法采用卡介苗(BCG)联合脂多糖(LPS)建立大鼠原代肝细胞免疫性损伤模型,测定并比较各组肝细胞培养上清液中谷丙转氨酶(ALT)、谷草转氨酶(AST)的活性和一氧化氮(NO)在不同培养时间点(3,6,12和24h)的含量。结果与模型组相比较,类叶升麻苷在不同时间点均能明显降低ALT、AST和NO水平(P<0.01),且呈剂量依赖关系。结论类叶升麻苷对卡介苗联合脂多糖诱导的大鼠原代肝细胞免疫性肝损伤具有保护作用。
Objective To study the effect of acteoside on immunological liver injury model in vitro. Methods Immunological liver inju- ry in rat primary hepatocytes was induced by Bacillus Calmette Guerin （BCG） plus lipopolysaccharides （LPS）. The levels of super- natant AST,ALT and NO at different cultural time points were determined by general methods. Results Compared with model group, acteoside （ 5,20 and 80 μg · mL-1 ） significantly decreased the supernatant enzyme activities （ AST, P〈0.01 ; ALT, P〈0.01 ） and nitric oxide [NO,P=0.01 ）. Conclusions The results indicate that aeteoside had significantly protective effects on the damage of the primary cultured hepatocytes in rats by BCG plus LPS.
出处
《西北药学杂志》
CAS
2013年第3期269-271,共3页
Northwest Pharmaceutical Journal
基金
国家自然科学基金项目(编号:81260624)
关键词
类叶升麻苷
肝细胞
免疫性肝损伤
肝保护作用
acteoside
hepatocytes
immunological liver injury
hepatoprotective effect
