摘要
目的以多指标筛选哮喘复方的纯化工艺。方法考察不同纯化工艺中有效成分的含量、制备的难易以及药效学进行评价。结果纯化工艺中以醇沉纯化效果最为理想,有效成分含量高且易制备;药效学评价在整体动物药物引喘法、豚鼠离体气管螺旋条法和小鼠氨水引咳法中醇沉纯化工艺效果最理想。结论醇沉纯化工艺为哮喘复方的最佳纯化方法,可用于该复方的进一步开发。
Objective To optimize the purification technology of Asthma Compound with chemical index and pharmaeodynamics index. Methods Four samples of asthma compound were prepared by different purification technology. The content of ephedrine, and pseudoephedrine was determined in Asthma Compound by HPLC. Their antiasthmatic and antitussive effects were also observed. Results The sample purified by alcohol precipitation had satisfactory results, high content of effective components has achieved by this method, and the effective components showed satisfactory antiasthmatic and antitussive effects. Conclusion The method of sample purification processed by alcohol precipitation is an optimal approach for purification, and could be used for the further development of the Asthma Compound.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2013年第3期312-316,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
十二五国家重大新药创制专项(2011ZX09102-011-01)
广东省科技厅(2011A030100009)
关键词
纯化工艺
药效学评价
麻黄碱
伪麻黄碱
Purification technology
Pharmacodynamics
Ephedrine
Pseudoephedrine
