摘要
目的:考察阳离子交换树脂不同洗脱溶剂纯化长春花中文多灵和长春质碱的效果,优选最佳工艺条件。方法:采用LSD-001大孔型阳离子交换树脂对长春花中文多灵和长春质碱进行纯化,以两种生物碱含量为指标,考察以氨水、氨性乙醇、酸水、酸性乙醇、盐醇为洗脱剂纯化两种生物碱的效果。结果:经过优选,离子交换树脂的最佳洗脱条件为:以80%乙醇(含1%氯化钠)洗脱剂洗脱时,两种生物碱的洗脱率最高。结论:优选得到的工艺纯化长春花中文多灵和长春质碱效果好,节省溶剂,工艺简单,可用于长春花中两种生物碱的分离纯化。
Objective：To select the optimum purifying process of Vindoline and Catharanthine from Catharanthus roseus by means of cation exchange resin.Method：The purifying process of Vindoline and Catharanthine from Catharanthus roseus was performed by cation exchange resin of LSD-001 type,with the solvent of ammonia water,the mixture of ammonia water and ethanol,acid water,the mixture of acid water and ethanol,the mixture of sodium chloride and ethanol,and the content of two alkaloids were determined to choose the optimal technological parameters.Result：The ideal concentration of solvent was：with 80% ethanol（containing 1% sodium chloride）eluent elution,two alkaloids eluting attained the highest rate.Conclusion：The purification process has well effect.It can be used for purifying Vindoline and Catharanthine from Catharanthus roseus by means of cation exchange resin.
出处
《辽宁中医药大学学报》
CAS
2012年第12期182-184,共3页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
阳离子交换树脂
长春花
文多灵
长春质碱
纯化工艺
cation exchange resin
Catharanthus roseus
Vindoline
Catharanthine
purifying process
