摘要
目的测定参萸颗粒中人参皂苷Rg1、Re含量。方法采用高效液相色谱法检测参萸颗粒中人参皂苷Rg1、Re含量。采用Dikma C18(5μm,200 mm×4.60 mm)柱;以乙腈-0.1%磷酸水溶液(20∶80,V/V)为流动相,检测波长为203 nm,流速为1 ml/min,进样量20μl,柱温为室温。结果人参皂苷Rg1在0.168-1.68 mg/ml浓度范围内、人参皂苷Re在0.295-1.475 mg/m浓度范围内呈良好线性关系,r分别为1.000和0.999 9;人参皂苷Rg1,Re的回收率(n=3)分别为98.38%(RSD=0.89%),97.21%(RSD=1.87%)。结论本方法准确、快速、重现性好,可用于参萸颗粒的质量控制。
Objective To establish a HPLC method for determining the contents of ginsenoside Rg1 and ginsenoside Re in Shenyu granule. Methods Dikma C18 column（250 mm ×4.6 mm,5 μm） was applied with the mobile phase of acetonitrile -0.1 phosphate acid water（20： 80） and the flow rate of 1.0 ml/min. The detective wavelength was set at 203 nm. The calibration curve method was in- vestigated to determine the contents of ginsenoside Rgl and ginsenoside Re. Results Ginsenoside Rgl and ginsenoside Re showed a good linear relationship between the concentration and the peak area in the range of O. 168 - 1.68 mg/ml and O. 295 - 1. 475mg/ml ,re- spectively. The average recovery was 98.38% （RSD =0.89% ） and 97.21% （ RSD = 1.87% ） ,respectively. Conclusion The estab- lished method is sensitive and repeatable, and can effectively control the quality of Shenyu granule.
出处
《山西医科大学学报》
CAS
2013年第4期288-290,共3页
Journal of Shanxi Medical University
基金
太原市2012年大学生创新创业专题基金资助项目(120164067)
