摘要
目的采用反相高效液相色谱法同时测定益智宁颗粒中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb13种皂苷的含量。方法采用Agilent Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm),流动相为乙腈-水,梯度洗脱:19%乙腈(0-35 min),19%乙腈-28%乙腈(35-50 min),28%乙腈(50-60 min),28%乙腈-29%乙腈(60-75 min),29%乙腈(75-90 min),29%乙腈-40%乙腈(90-100 min);流速:0.7 mL/min;检测波长:203 nm,柱温:25℃。结果人参皂苷Rg1、人参皂苷Re及人参皂苷Rb1的进样量分别在0.480-2.40μg、0.420-2.10μg和0.410-2.05μg范围内与峰面积呈良好的线性关系,相关系数r分别为0.9995、0.9999、0.9998;人参皂苷Rg1和人参皂苷Re总量的平均回收率为98.93%;人参皂苷Rb1的平均回收率为101.1%。结论本方法准确、灵敏度高、重现性好,可作为益智宁颗粒的质量控制方法。
Objective To establish a method to determine the contents of ginsenoside Rg1、ginsenoside Re and ginsenoside Rb1 in Yizhining Granules by HPLC.Methods The separation was preformed on Agilent Eclipse XDB-C18 column（4.6mm×150mm,5 μm）,The mobile phase consisted of acetonitrile-water with gradient elution： 19% acetonitrile（0-35 min）,19% acetonitrile-28% acetonitrile（35-50 min）,28% acetonitrile（50-60 min）,28% acetonitrile-29% acetonitrile（60-75 min）,29% acetonitrile（75-90 min）,29% acetonitrile-40% acetonitrile（90-100 min）,the flow rate： 0.7 mL/min,the wavelength of detector： 203 nm,the temperature of column： 25℃.Results The calibration curves showed good linearity in the range of 0.480-2.40 μg（ginsengnoside Rg1,r=0.9995）、0.420-2.10 μg（ginsengnoside Re,r=0.9999）and 0.410-2.05 μg（ginsengnoside Rb1,r=0.9998）.The average recoveries of ginsengnoside Rg1 and ginsengnoside Re is 98.93%.The average recoveries of ginsengnoside Rb1 is 101.1%.Conclution The method is accurate,hypersensitized 、reproducible,which can be applied to the quality of Yizhining Granules.
出处
《湖北中医药大学学报》
2012年第2期25-27,共3页
Journal of Hubei University of Chinese Medicine
