摘要
目的:建立同时测定消臌胶囊中没食子酸和橙皮苷的HPLC法,为该中药复方制剂的质量控制提供一种简便、快速、准确的方法。方法:采用甲醇加热回流提取消臌胶囊,选取Synergi 4μHydro-RP 80A色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.04 mol.L-1磷酸二氢钠溶液(20∶80),流速1.0 mL.min-1,检测波长283 nm,柱温25℃。结果:在此色谱条件下,没食子酸和橙皮苷达到基线分离,分别在21.6～216.0,4.5～45.0 mg.L-1与峰面积成良好的线性关系,平均回收率(n=9)分别为101.5%(RSD 3.7%),94.7%(RSD 2.7%)。消臌胶囊中没食子酸和橙皮苷的平均含量分别为5.10%,0.091 1%。结论:该研究建立的方法能快速准确地测定消臌胶囊中没食子酸和橙皮苷的含量,可为该药的质量评价提供参考。
Objective： To develop an HPLC method for simultaneous determination of gallic acid and hesperidin in Xiaogu capsule, in order to provide a simple, rapid and accurate method for quality control of the compound preparation of traditional Chinese medicine. Method： Xiaogu capsule was extracted with methanol heating reflux method. Synergi 4μ Hydro-RP 80A （4. 6 mm × 250 mm,5 μm） was adopted as the chromatographic column, with acetonitrile-0. 04 mol ~ L-1phosphate monobasic sodium solution （20： 80） as the mobile phase. The flow rate was 1.0 mL· min^- 1, the detection wavelength was 283 nm, and the column temperature was 25 ℃. Result： Under the conditions, gallic acid and hesperidin reached the baseline resolved peak, with a good linearity within the range of 21.6-216.0 mg· L^-1 （ r = 0. 999 93 ） for gallic acid, and 4. 5-45.0 mL·min^-1 （ r = 0. 999 95 ） for hesperidin, respectively. Their average recoveries （ n = 9） were 101.5% （ RSD 3.7% ） and 94. 7% （ RSD 2. 7% ）, respectively. The average contents of gallic acid and hesperidin contained in Xiaogu capsule were detected to 5.10% and 0. 091 1%, respectively. Conclusion： The method established in this study can determine the content of gallic acid and hesperidin contained in Xiaogu capsule in a rapid and accurate manner, which provided reference for quality evaluation of the medicine.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第10期1518-1520,共3页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973)项目(2011CB935800)
吉林省科技厅重点项目(20100905)
吉林省高技术产业发展项目(2010014)
