摘要
目的:建立HPLC同时测定小儿金宁口服液中绿原酸、隐绿原酸、咖啡酸、柚皮苷、橙皮苷、蒙花苷6个成分的方法。方法:Lichrospher C18柱(4.6 mm×250 mm,5μm);以乙腈(A)-0.4%磷酸水溶液(B)为流动相进行梯度洗脱(10∶90～18∶82～27∶73),流速(0.8～1.1～0.8)mL.min-1,柱温30℃,检测波长300 nm。结果:6种成分均能达到基线分离,各成分都有较宽的线性范围和良好的线性关系,加样回收率在95%～105%。结论:本法快速、准确、可靠、重复性好,可为小儿金宁口服液的质量控制提供参考依据。
Objective： To develop a HPLC method for the simultaneous determination of chlorogenic acid,cryptochlorogenic acid,caffeic acid,naringin,hesperidin and linarin in Xiao′ erjinning oral liquid.Method： The chromatographic separation was achieved on a Lichrospher C18（4.6 mm×250 mm,5 μm）column with a mobile phase which was composed of acetonitrile（A）and 0.4% phosphoric acid（B） for gradient elution（10∶90-18∶82-27∶73）.The flow rate was（0.8-1.1-0.8）mL·min-1,the column temperature was 30 ℃ and the detection wavelength was set at 300 nm.Result： The results showed that 6 effective components were separated well and showed good linearity.The average recoveries were between 95%-105%.Conclusion： The method is proved to be rapid,accurate,credible and repeatable.It can be used for the quality control of Xiao′erjinning oral liquid.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第13期1702-1705,共4页
China Journal of Chinese Materia Medica
基金
江苏省"青蓝工程"科技创新团队支持计划
中医儿科重点学科项目(EZK2009019)
关键词
绿原酸
隐绿原酸
咖啡酸
柚皮苷
橙皮苷
蒙花苷
chlorogenic acid
cryptochlorogenic acid
caffeic acid
naringin
hesperidin
linarin
