摘要
为评价莠去津在土壤中的遗传毒性,以赤子爱胜蚓(Eiseniafoetida)为研究对象,以质量比为0 mg/kg、0.1 mg/kg、0.5 mg/kg、2.5mg/kg莠去津对蚯蚓进行暴露染毒,在第7 d、14 d、21 d、28 d、35 d,利用彗星实验技术,取其DNA尾长和尾部DNA百分数作为DNA损伤的指标,研究了莠去津对蚯蚓体腔细胞DNA的损伤。结果表明:1)0.1mg/kg及以上质量比莠去津持续暴露35 d均会对赤子爱胜蚓体腔细胞DNA产生影响,与对照组相比,暴露染毒组体腔细胞DNA均受到损伤且损伤存在显著性差异(p<0.01);2)在长期暴露条件下,0.1～2.5 mg/kg莠去津对赤子爱胜蚓体腔细胞DNA的影响具有明显的剂量-效应关系;3)同一质量比莠去津处理下,暴露时间的延长增加了蚯蚓体腔细胞DNA的损伤程度;4)相同处理时间下,不同质量比莠去津处理组间差异显著(p<0.01)。
This present paper is aimed to introduce our investigation of the DNA damage caused by the atrazine in the body of earthworms （ Eisenia foetida ） through single-cell gel electrophoresis （ SCGE ）. The so-called single-cell gel electrophoresis, is also named comet assay, a rapid, simple and sensitive technique for measuring DNA strand which may get broken in the individual cells with ecotoxicology of atrazine （ 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine ） in the soil. As is known, improper use of pesticides may lead to one of the most important environmental pollution consequences in the soil. In order to assess the pollution situation, general practice is to add atrazine to artificial soil at the rates of 0 mg/kg, 0.1 mg/kg, 0.5 mg/kg and 2.5 mg/kg of soil. Then, earthworms exposed to each treatment could be collected and tested on the 7th, 14th, 21st, 28th and 35th day in the treatment process. In so doing, the following results can be found： （ 1 ） Single coelomocytes cells of earthworms were found suspended; （2） The slides were made in three levels; （3） Electrophoresis was done in which the slides could be observed under a fluorescent microscope. Compared with the contrast group, length of the worms and the tail of DNA% of the comet assay of coelomocytes, was detected to increase after the treatment with the increase of the doses of atrazine as the parameter of DNA damages on the 7th, 14th, 21st, 28th and 35th day, though significant difference was found with the contrast group （p 〈 0.01）. Thus, it can be concluded from our investigation that it would be possible to assess the DNA damage in earthworm bodies induced by atrazine by using comet assay through the following indications： （1） 0. 1 mg/kg atrazine can induce DNA damages on earthworms; （2） There was a positive relation of reply-dosage for all concentrations of atrazine tested; （3） DNA damages was found to increase as the exposure time prolonging at the same atrazine dose; （4） During the same length of treatment time, significant difference can be found at the different doses of atrazine. （5） Serious concern should be paid to the potential hazards to protecting terrestrial organisms with great need to renew agricultural practice in using pesticides However, further studies prove to be of urgent need to study further the biological consequences of DNA damage in this way.
出处
《安全与环境学报》
CAS
CSCD
北大核心
2009年第5期24-29,共6页
Journal of Safety and Environment
基金
国家自然科学基金项目(20477022
40801203)
中国博士后科学基金项目(20080431215)
