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畜禽沙门氏菌药敏性研究及两种测定方法比较 被引量:3

Study on Susceptibility of Salmonella from Livestock and Poultry to Drugs and Comparison of Two Testing Methods
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摘要 采用Kirby-Bauer纸片扩散法(以下简称K-B法)和梅里埃公司的ATB Expression自动细菌鉴定及药敏分析仪(以下简称ATB法),测定了36株畜禽源沙门氏菌对16种抗生素的敏感性。结果表明:ATB法和K-B法对阿莫西林/克拉维酸、亚胺培南和复方新诺明的测定结果有显著性差异(P<0.05),对其他13种药物的测定结果没有显著性差异;全部菌株耐1种以上的抗生素,58%的菌株耐2种以上的抗生素。得出以下结论:在畜禽源沙门氏菌药敏性研究中,ATB法还不能完全替代K-B法;畜禽源沙门氏菌分离菌株不仅耐药性较强,而且耐药谱也在不断扩大。 The susceptibilities of 36 Salmonella strains isolated from livestock and poultry products to 16 antibiotics were tested by using Kirby - Bauer slip - diffusion method ( K - B method) and the method of BioMerieux ATB Expression automatic identifica- tion (ATB method). The statistical results showed that there were significant differences in the testing results of Salmonella suscep- tibility to Amoxicillin / Clavulanic acid, Imipenem and Sulfamethoxazole - trimethoprim between ATB method and K - B method (P 〈0.05), but no significant differences in the testing results were found between the two methods for other 13 antibiotics. All the i- solated strains were resistant to one or more antibiotics, and 58% of the strains were resistant to two or more antibiotics. In summary, ATB method still could not completely replace K - B method in the studies on the drug susceptibility of Salmonella strains derived from livestock and poultry. The isolated strains had stronger drug resistance, and their drug - resistant spectrum also continued to expand.
出处 《江西农业学报》 CAS 2013年第5期114-116,119,共4页 Acta Agriculturae Jiangxi
基金 农业部公益性行业(农业)科研专项(200903055) 江西省卫生厅重大科研项目(20104015)
关键词 畜禽产品 沙门氏菌 药敏性 K-B法 ATB法 Livestock and poultry products Salmonella Drug susceptibility K - B method ATB method
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