摘要
目的：为了解河南省生肉食品中沙门菌（Salmonella）的污染和耐药状况,建立河南省食源性沙门菌DNA指纹图谱库。方法：采用Biolog全自动微生物分析系统对分离可疑菌株进行鉴定;K-B氏纸片法进行药敏试验;Ribo-PrinterMicrobial Characterization System测定沙门菌的DNA指纹图谱。结果：399份生肉样品中,118份检出沙门菌,总体检出率为29.57%。2007年的检出率高于2006年,结果差异无显著性（χ^2=0.18,P〉0.05）。鸡肉样品中沙门菌检出率（38.33%）高于猪肉（16.35%）,二者差异有显著性（χ^2=88.69,P〈0.05）。对121株沙门菌进行了12种抗生素的药敏试验,总体上受试菌株对12种抗生素均表现耐药,其中对阿米卡星耐药率最低为1.69%,对萘啶酮酸耐药率最高,达88.14%;比较沙门菌对12种抗生素的耐药性,2007年的耐药菌株比2006年多,并发现沙门菌的ESBL株7株,AMPC株2株,沙门菌耐药的广度和深度均有明显上升的趋势。对2006-2007年自河南省市售生肉食品中分离得到的121株沙门菌菌株进行了DNA指纹图谱的测定,初步建立了河南省食源性沙门菌的DNA指纹图谱库。结论：监测结果表明,河南省市售生肉食品沙门菌污染严重,且分离菌株对常用抗生素表现出广泛的耐药性,对公众健康构成一定的威胁。建议加强家畜家禽饲料添加剂管理和人类抗生素使用管理,以保护人类的抗生素资源。
Objective： To investigate the situation of Salmonella contamination and antibiotic resistance in raw meat foods and establishing the foodborne Salmonella DNA fingerprint database in Henan province. Methods：The putative Salmonella strain was confirmed by Biolog automated microbial analysis system, the antibiotic susceptibility was tested by using Kirby - Bauer paper flakes diffusion method and the Salmonella DNA fingerprint database was determined through Ribo - Printer Microbial Characterization System. Results：Salmonella was detected in 118 of 399 raw meat food samples which showed the detection rate was 29. 57%. The detection rate in 2007 was higher than that in 2006 but no significant difference between two years （X^2 =0. 18 ,P 〉 0. 05）. Salmonella detection rate in chicken （38.33%） was higher than that in perk （16. 35% ） and showed significant difference （X^2 = 88. 69, P 〈0. 05）. 12 antibiotics were employed to determine the susceptibility of 121 Salmonella and most of strains showed resistance to the antimicrobials used in the test, the amikacin resistance rate （ l. 69% ） was the lowest in all antibiotics, resistance rate to nalidixic acid was the highest, which reached to 88. 14%. The antibiotic resistant Salmonella strains in 2007 were much more than that in 2006 to above antimicrobials, 7 ESBL and 2 AMPC Salmonellas were found, both the breadth and depth of Salmonella to antibiotic resistance had a obvious upward trend. The DNA fingerprint of 121 Salmonella strains isolated from raw meat food in Henan province in 2006 - 2007 was determined and the DNA fingerprint database of foodbome Salmonella in Henan province was established preliminarily. Condusion： Inspection results showed that Salmonella contamination in raw meat foods was serious in Henan province, the isolates expressed extensive resistance to the antibiotics that were commonly used in clinical therapy and have had a threat to public health. The advice was proposed to strengthen the management of additives of livestock and fowl as well as the antimicrobials used in human to protect the antimicrobial resources.
出处
《中国卫生检验杂志》
CAS
2009年第7期1545-1548,共4页
Chinese Journal of Health Laboratory Technology
基金
河南省医药卫生科技攻关重点项目(20060150)
关键词
生肉食品
沙门菌
药敏试验
DNA指纹图谱
Raw meat food
Salmonella
Antibiotic susceptibility test
DNA fingerprints
