摘要
目的：探讨吗啡对小鼠血清一氧化氮（ＮＯ）的影响。方法：①吗啡急性用药实验：将小鼠随机分为５组 （每组 １０只），其中４组按 １００ｍｇ／ｋｇ腹腔注射盐酸吗啡，将小鼠在注射吗啡后 ０．５ ｈ，１ｈ，２ ｈ，４ ｈ分批处死并收集血 清，另１组注射生理盐水为对照组，在注射后１ｈ处死小鼠并收集血清。②吗啡成瘾性实验：另将小鼠随机分为５ 组（每组 １０只），其中４组每 ｄ按 １００ｍｇ／ｋｇ注射盐酸吗啡，共注射 １０ ｄ，在末次注射后６ ｈ分别将吗啡成瘾组、纳络 酮诱发戒断组小鼠处死并收集血清，其余２组小鼠在自然戒断１ｄ及６ｄ后处死并收集血清；另１组注射生理盐水 为对照组，每 ｄ按０．５ ｍｌ／只注射生理盐水，共注射 １０ ｄ，在末次注射后 ６ ｋ后处死小鼠并收集血清。③采用硝酸还 原酶法检测血清 ＮＯ浓度。结果：①吗啡急性用药实验：在注射吗啡后 ０．５ ｈ，ｌｈ，２ ｈ血清 ＮＯ浓度分别为（１２１．０９ ±１６.６６）μｍｏｌ／Ｌ（１３２．００±２７.５１）μｍｏｌ／Ｌ，（１２７．３５±１３．２９）μｍｏｌ／Ｌ，均显著高于对照组（８６．６２±７．８７）μｍｏｌ／Ｌ，（Ｐ＜ ０．０１），４ ｈ的小鼠血清ＮＯ浓度为（９４．６３±７．８２）μｍｏｌ／Ｌ，?
Aim: To determine the effects of morphine hydrochloride on mice serum nitric oxide(NO) level. Methods: ① Tests of acute injection of morphine:Mice were divided into five groups (10 of each group). Four groups were injected with mor- phine hydrochloride at dose of 100 mg/kg and serum was collected at 0.5 h, 1 h,2 h and4 h for NO examination. The other group was injected with normal saline as control and serum was collected at 1h for NO examination. ②Tests of morphine addiction:Other mice were divided into five groups(10 for each group). Four were injected with morphine hydrochloride at the dose of l00mg/kg for 10 days so that the mice were addicted to morphine .The other group was injected with normal saline at the dose of 0.5 ml per mouse for 10 days as control. Serum of morphine addicted group,morphine abstinece group were collected after 6h of the last in- jection. Serum of the spontaneousness abstinence 1d and 6d groups were the collected. Serum of the control group was collected after 6 h of the last injection. ③NO were examined by using nitric acid reductase method. Results: ①Tests of acute injection of morphine:The values of serum NO were (121 .09±16.66)μmol/L, (132.00±27.51)μmol/L and (127.35±13.29)μmol/L at 0.5 h, 1 h and 2 h respectively after morphine injection, and of control group was (86.62±7.87)μmol/L.There were statistically difference between each experimental all control group(P < 0.01) .②Tests of morphine addiction: The values of serum NO of morphine addicted group, morphine abstinence group and spontaneousness abstinence 1 d group were (114.60±15.15)μmol/ (122. 12±22. 73) μmol/L and (132. 81±39. 88)μmol/L, which were higher than that of control group (92. 97± 14. 98) μmol/L respectively (P<0. 05, P<0. 01, P<0. 01), and that of the spontaneousness abstinence 6 d group had no difference compared with that of control group. Conclusion: The levels of serum NO raised after morphine injection, morphine addiction and morphine abstinence.
出处
《河南医科大学学报》
2000年第5期422-424,共3页
Journal of Henan Medical University
基金
河南省“九五”重大攻关项目971200600
关键词
吗啡类
一氧化氮
行为
成瘾
小鼠
morphine hydrochloride
nitric oxide
addition
mice
