摘要
目的 :观察滤泡性淋巴瘤 (FL)与反应性淋巴滤泡增生 (RFH)中滤泡生发中心细胞 (GCCs)核DNA含量、倍体情况以及bcl 2癌基因蛋白、免球蛋白轻链 (IgL)表达情况 ,探讨它们对于二者鉴别诊断的意义。方法 :对 2 1例FL及 2 1例RFH进行bcl 2蛋白、Kappa、Lambda轻链蛋白免疫组化检测及DNA图像细胞分析。结果 :6 1 9%FL中GCCs有bcl 2表达而RFH的GCCs均为bcl 2阴性 (P <0 0 0 5 )。IgL限制性表达仅在 6 / 12中有满意检出。肿瘤性GCCs的DNA含量显著高于正常增生性GCCs(P <0 0 0 1) ,6 6 7%的FL病例为超二倍体而 90 5 %RFH系二倍体或亚二倍体。FL各细胞学分级之间bcl 2表达率及DNA含量有所差异。结论 :DNA定量分析与bcl 2检测两方法有互补作用 ,对于FL与RFH鉴别诊断及预后估计有实用意义 。
Purpose:To investigate DNA content, DNA ploidy status and the expression of bcl 2 oncoprotein, immunoglobulin light chains(IgL) in follicular germinal center cells (GCCs) of follicular lymphoma (FL) and reactive follicular hyperplasia(RFH).Methods:Twenty one cases of FL and the same number cases of RFH were subjected to DNA image cytometry. And bcl 2 protein,Kappa,Lambda light chains were immunohistochemically detected. Results:The bcl 2 positivity of GCCs in FL and RFH were 61.9% and 0%,respectively ( P <0 005). And the restrictive expression of IgL was only seen in 6/12 Fl cases. Neoplastic GCCs have significantly higher mean DNA content than hyperplastic ones ( P <0 001). 66.7% FL cases were hyperdiploid while 90.5% RHF were diploid or hypodiploid. The variation of bcl 2 positivity and DNA content between different cytological grades of FL was noticed,too. Conclusions:Quantitative DNA analysis by image cytometry combined with bcl 2 immunohistochemical detection can be very helpful to the distinction between FL and RFH.
出处
《中国癌症杂志》
CAS
CSCD
2000年第3期207-210,共4页
China Oncology
关键词
滤泡性淋巴瘤
反应性淋巴滤泡增生
DNA图像分析
Follicular lymphoma
Reactive follicular hyperplasia
Differenital diagnosis
bcl-2 oncoprotein
Immunoglobulin light chain restriction
Immunohistochemistry
DNA image cytometry
