摘要
目的采用细胞图像分析术(ICM)对非霍奇金淋巴瘤(NHL)进行DNA含量、S期分数(SPF)的检测,探讨两个参数在NHL诊断分型、鉴别诊断及预后判断中的意义。方法210例NHL和30例良性增生淋巴组织进行改良Feulgen染色和ICM-DNA检测。结果NHL的DNA含量为(7.01±0.21)pg～(10.77±0.27)pg,SPF为(10.97±1.11)%～(30.87±2.17)%,均高于对照组(P<0.05);NHL侵袭性、高侵袭性组的DNA含量和SPF均高于惰性组(P<0.05);滤泡性淋巴瘤FL-II、III级的DNA含量、SPF均高于FL-I级(P<0.05)。结论DNA含量、SPF对良恶性淋巴组织增生的鉴别诊断、NHL惰性或侵袭性判断是有意义的参考指标,但对侵袭性高低无提示;FL是相对异质化群体,DNA参数检测只能做为参考。
[Objective] To investigate the diagnostic and prognostic significance of DNA content and S-phase fraction （SPF） by DNA image cytometry analysis （ICM-DNA） in non-Hodgkin＇s lymphoma （NHL）. [Methods] ICM-DNA was performed on 210 NHL and 30 benign lymphoid hyperptasia specimens prepared by Feulgen stain. DNA content and SPF were computed. [Results] DNA content [（7.01±0.21） pg - （10.77 ±0.27） pg] and SPF [（10.97 ± 1.11）% - （30.87±2.17）%] in NHL was higher than in benign lymphoid hyperplasia （P 〈0.05）. There is significant difference between indolent and aggressive, indolent and highly aggressive, FL-I and FL-II, FL-I and FL-m （P 〈 0.05）, but nonsignificant difference between aggressive and highly aggressive, FL-II and FL-III in DNA content and SPF （P 〉0.05）. [Conclusion] DNA content and SPF can be useful in distinguishing between malignant and benign lymphoid hyperplasia, indolent and aggressive/highly aggressive, but neither of them could differentiates between aggressive and highly aggressive. FL is heterogeneous, DNA content and SPF detection can be just for reference.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第12期1797-1800,共4页
China Journal of Modern Medicine
基金
广东省医学科研基金项目(No:B2006073)
广州医学院自然科学科研项目(No:2006ZR014)
关键词
非霍奇金淋巴瘤
DNA含量
S期分数
non-hodgkin＇s lymphoma （NHL）
DNA content
S-phase fraction （SPF）
