摘要
为更客观地评价蚕蛹抗氧化肽的抗氧化能力,以人工胃肠模拟液对不同蛋白酶酶解物的抗氧化能力进行评价,优选获得最佳的制备用酶——碱性蛋白酶.在此基础上,以蚕蛹抗氧化肽总还原能力为指标,利用响应曲面法(response surface method,RSM)优化碱性蛋白酶制备蚕蛹抗氧化肽的最佳酶解工艺.结果表明:碱性蛋白酶的最佳酶解条件为pH=8.30,加酶量为15.34μLL(1 902 U),温度为50.47℃,时间为2.45 h;在此条件下优化预测得到的2 mg蚕蛹蛋白/mL酶解液的总还原能力为0.434,蚕蛹抗氧化肽对·OH和DPPH·自由基的半数清除率IC_(50)分别为4.51与4.24 mg/mL.
Alkaline protease was chosen by the effect of gastrointestinal fluid simulation which was used to predict the activity in vivo of silkworm pupae protein. Response surface method （RSM） was employed to optimum the optimal alkaline protease enzymolysis procedure of silkworm pupae protein for antioxidant peptides with high reducing power. Antioxidant activity of enzymolysates was assessed with reducing power and scavenging effects against · OH and DPPH · radicals. Results show that the optimum conditions are as follows： pH at 8.30, quantity of alkaline protease at 15.34 μL（1 902 U）, temperature at 50.47 ℃ and time at 2.45 h. Under the optimum conditions, the predicted total reducing power of 2.0 mg silkworm pupae protein/mL enzymolysates was 0.434. On the basis of the optimum conditions of the enzymolysates, the half-scavenging concentration IC50 values of ·OH and DPPH · radicals were 4.51 and 4.24 mg/mL respectively.
出处
《上海大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第2期215-220,共6页
Journal of Shanghai University:Natural Science Edition
基金
上海市教委重点学科建设资助项目(J50108)
上海高校选拔培养优秀青年教师科研专项基金资助项目(SHU09063)
关键词
响应曲面法
蚕蛹蛋白
酶解
抗氧化肽
response surface method （RSM）
silkworm pupae protein
enzymolysis
antioxidant peptides
