摘要
目的探讨心肌注射单宁酸(TA)对心肌梗死(MI)后心肌细胞外基质(ECM)重构的影响。方法建立大鼠心肌梗死模型,将60只大鼠随机分为单宁酸组(n=30),对照组(n=30)。术后,用超声心动图评价心功能;术后4周,检测左、右心室重量指数,观察心肌梗死边缘区组织心肌细胞外基质(ECM)微结构;术后2周和4周,测定心肌组织胶原蛋白含量。结果符合实验要求的大鼠心梗模型成功率为70%左右;单宁酸组的心功能明显好于对照组(P<0.05);术后2周,单宁酸组的心梗区胶原蛋白含量高于对照组(56.75±6.78 vs 41.25±4.76,P<0.05);术后4周,单宁酸组左、右心室重量指数低于对照组(P<0.05);单宁酸心肌注射明显改善ECM排列结构。结论在MI急性期,心肌注射TA可通过交联胶原,抑制胶原的降解,减轻心肌ECM重构。
Objective To investigate the effects of tannic acid（TA） on cardiac extracellular matrix（ECM） remodeling after acute myoeardial infarction. Methods Myocardial infarction model in rats was induced by left coronary artery ligation. Sixty rats were enrolled and divided into tannic acid intramyocardial injection group （ TA group, n = 30 ） and saline solution intramyocardial injeetion group（ control group, n ： 30）. Heart function was evaluated by echocardio- graphy 24 hours after myocardial infarction and 2nd and 4th week after injection. Four weeks after injection,the structure of cardiac extracellular malrix（ECM） in the infarcted borderline area was evaluated. Left ventricular weight index （LVWI） and right ventricular weight index （RVM1） were detected respectively. Collagen content in the infarcted area was evaluated by hydroxyproline colorimetry assay at 2nd and 4th week after injection. Results The arrangement of ECM was effectively improved and LVWI and RVMI was decreased in TA group after intramyoeardial injection. Compared with control group,the collagen content was increased after intramyocardial injeetion of tannic acid at the early postinfarction stage （ 56.75mg/g ± 6.78mg/g,41.25mg/g± 4.76rag/g, P 〈 0.05 ）. After injection, heart function of the rats was better in TA injection group than in control group. Conclusions Tannic acid after local intramyocardial injection prevents col- lagen matrix degradation via cross-linking fibrous collagen, and therefore, effectively attenuate ECM remodeling after AMI.
出处
《潍坊医学院学报》
2013年第2期113-116,共4页
Acta Academiae Medicinae Weifang
关键词
心肌梗死
单宁酸
心室重构
细胞外基质
Myocardial infarction
Tannic acid
Myocardial remodeling
Cardiac extracellular matrix
