摘要
目的探讨阿托伐他汀对大鼠心肌梗死(myocardial infarction,MI)后心肌细胞核内FoxO3a表达和心室重构的影响。方法建立大鼠MI模型,24 h后存活大鼠随机分成MI组(n=8)、阿托伐他汀10 mg组[10 mg/(kg.d),Ato组,n=8],同时另设假手术组(Sham组,n=10)。4周后观察左心室质量指数(left ventricular mass index,LVMI),免疫组化染色和RT-PCR检测FoxO3a在左心室非梗死区(non-infarction zone,NIZ)心肌细胞核内蛋白质和mRNA表达水平,流式细胞技术(flow cytometry,FCM)检测FoxO3a蛋白在NIZ心肌细胞核内表达含量。SAS9.1统计软件分析数据。结果 MI组与Sham组相比,LVMI显著增加(P<0.05);左室心肌非梗死区FoxO3a mRNA、FoxO3a蛋白表达(免疫组化)、FCM检测心肌细胞核内蛋白表达量表达均降低(P<0.05)。与MI组相比,Ato组LVMI显著下降(P<0.05);但高于Sham组(P<0.05);与MI组比较Ato组左室心肌非梗死区FoxO3a mRNA、Fox-O3a蛋白表达(免疫组化)、FCM检测心肌细胞核内蛋白表达量表达均显著增高(P<0.05);但低于Sham组(P<0.05)。结论阿托伐他汀能够有效地改善MI后心室重构,机制可能与上调细胞核内FoxO3a表达量有关。
We aimed to assess the effects of atorvastatin on ventricular remodeling in rats after myocardial infarction and to investigate the alternation of the expression of FoxO3a in the nuclear of myocardial cells.Twenty-four hours after myocardial infarction by left anterior descending coronary artery ligation,we randomly divided the survival rats into myocardial infarction group（MI,n = 8）,atorvastatin 10 mg/（kg·d） treatment group（Ato,n = 8）,at the same time we established sham-operated animals underwent identical surgery except for the coronary artery ligation（Sham,n = 10）.Four weeks after,we evaluated the effects of atorvastatin on myocardial fibrosis by detecting the changes of left ventricular mass index（LVMI）,and measured the expressions of FoxO3a in non-infarction zone（NIZ） by immunohistochemistry staining and reverse transcription polymerase chain reaction（RT-PCR）.We also measured the levels of non-Phosphorylation FoxO3a by flow cytometry（FCM）.All data were analyzed the data by SAS 9.1 software.Compared with Sham group,the LVMI was significantly increased in MI group（P〈0.05）,but the expression level of FoxO3a mRNA as well as the FoxO3a expression level detected by immunohistochemistry and FCM in NIZ were both significantly decreased in MI group.Compared with MI group,the LVMI was significantly decreased（P〈0.05） in Ato group,but higher than those in Sham group（P〈0.05）.The expressions of FoxO3a both in mRNA level and in protein level were significantly increased in Ato group（P〈0.05）,but lower than those in Sham group（P〈0.05）.According all these data,we conclude that atorvastatin can ameliorate ventricular remodeling in rats induced by MI,and the mechanisms may be associated with the up-regulating of FoxO3a.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2011年第5期381-385,共5页
Immunological Journal
基金
重庆市卫生局医学科学技术项目(2010-01-07)
重庆医科大学附属第一医院重点项目基金(2009-07)
