摘要
目的探讨白介素1受体辅助蛋白(interleukin 1 receptor accessory protein,IL-1RAP)在成人与儿童低级别胶质瘤中的表达,分析其与预后的相关性。方法应用高通量全基因分析、免疫组织化学法、qRT-PCR和Western blotting方法对20例低级别胶质瘤(成人10例,儿童10例)标本中IL-1RAP的表达进行检测。结果经高通量全基因分析、免疫组织化学、qRT-PCR和Western blotting分析证明IL-1RAP与成人比较,儿童低级别胶质瘤总阳性表达率较低,有统计学意义(100%>30%,Chi-Square=12.439,P<0.05)。结论 IL-1RAP可能在胶质瘤的发生、发展过程之中扮演重要的角色。IL-1RAP可能成为评价低级别胶质瘤预后差异的重要参考指标及潜在的药物靶点。
Objective To analyze the expression of interleukin 1 receptor accessory protein（ IL-1 RAP） in children and adult low grade gliomas（LGG） and correlation between the level of this markers and low grade gliomas recurrence. Methods The samples of LGG were collected and divided into two groups（ adult group and children group）. Gene expression profiles in adult group and children group were compared by microarray. The expression of changed gene was indentified by immunohistochemistry（IHC） , Western blot and quantitative real-time PCR（qRT-PCR） in 20 cases of LGG （10 children, 10 adult）. Results We identified that IL-1RAP was consistently up- regulated in children group LGG with the adult group LGG by cDNA array, and the level of IL-1 RAP in children group was significantly higher than that of adult group detected by RT-PCR, Western blot and IHC （ Chi-Square = 12. 439, P〈0.05）. Conclusion IL-1 RAP may be the potential biomarkers to evaluate the inclination of invasion and recurrence in children and adult low grade gliomas. And the dysregulation of IL-1 RAP may be critical for malignant astrocytoma formation, and thus a possible novel universal target for therapeutic intervention.
出处
《首都医科大学学报》
CAS
2013年第2期258-263,共6页
Journal of Capital Medical University
基金
国家自然科学基金(81072074
31271119)
北京市教委科技计划重点项目(KZ201110025033)
北京市优秀人才基金(2011D003034000004)资助~~
关键词
白介素1受体辅助蛋白
低级别胶质瘤
成人
儿童
interleukin 1 receptor accessory protein （ IL-1 RAP）
low grade gliomas
adult
children
