摘要
背景:细胞外调节蛋白激酶和酸性成纤维细胞生长因子受体2在肠缺血-再灌注损伤修复中的作用尚无研究报道。目的:观察大鼠肠缺血-再灌注损伤后外源性酸性成纤维细胞生长因子对细胞外调节蛋白激酶和酸性成纤维细胞生长因子受体2表达的影响,探讨细胞外调节蛋白激酶和酸性成纤维细胞生长因子受体2与酸性成纤维细胞生长因子促进创伤修复的关系。方法:以大鼠肠系膜上动脉夹闭45min造成肠缺血-再灌注损伤模型,于再灌注即刻应用酸性成纤维细胞生长因子行干预。分别于再灌注2,6,12,24h取大鼠小肠组织标本,利用免疫组化和RT-PCR检测酸性成纤维细胞生长因子受体的表达及免疫组化检测细胞外调节蛋白激酶表达的规律。结果与结论:在正常大鼠,酸性成纤维细胞生长因子受体2主要分布在小肠绒毛上皮细胞的肠腔侧、侧壁和小肠隐窝朝向隐窝腔的一侧细胞膜上。缺血-再灌注初期,酸性成纤维细胞生长因子受体2及细胞外调节蛋白激酶的表达未发生明显变化,但随着再灌注时间的延长表达水平逐渐提高,并于再灌注后6-12h达高峰。经酸性成纤维细胞生长因子治疗后,大鼠小肠组织小肠黏膜损伤程度减轻,酸性成纤维细胞生长因子受体2及细胞外调节蛋白激酶的表达量高于未治疗大鼠。结果表明缺血-再灌注损伤后,酸性成纤维细胞生长因子干预可上调酸性成纤维细胞生长因子受体2及细胞外调节蛋白激酶的表达,提示外源性酸性成纤维细胞生长因子通过促进内源性酸性成纤维细胞生长因子受体2和细胞外调节蛋白激酶的生成可能是其参与内脏损伤修复的机制之一。
BACKGROUND： Rote of extraceltlular signal-regulated kinase and acidic fibroblast growth factor receptor 2 in intestinal ischemia-reperfusion injury repair has not been reported. OBJECTIVE： To identify the changes in extracellular signal-regulated kinase and acidic fibroblast growth factor receptor 2 regulated by exogenous acidic fibroblast growth factor （acidic fibroblast growth factor） following intestinal ischemia-reperfusion injury in rats, and to explore the role of extracellular signal-regulated kinase and acidic fibroblast growth factor receptor 2 in acidic fibroblast growth factor-induced injury repair. METHODS： Intestinal ischemia-reperfusion injury models were produced in rats by clamping the superior mesenteric artery for 45 minutes, and then acidic fibroblast growth factor administration was applied immediately after modeling. Tissue specimens were collected at 2, 6, 12, and 24 hours after reperfusion. The expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase were detected by immunohistochemistry and reverse transcription-PCR. RESULTS AND CONCLUSION： The expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase increased gradually after ischemia-reperfusion injury and peaked at 6-12 hours after reperfusion. Acidic fibroblast growth factor administration relieved intestinal mucosa injury and increased the expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase. These results suggest that acidic fibroblast growth factor administration can upregulate the expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase after ischemia-reperfusion injury, indicating exogenous acidic fibroblast growth factor can participate in visceral damage repair via the activation of endogenous acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase.
出处
《中国组织工程研究》
CAS
CSCD
2013年第11期1965-1971,共7页
Chinese Journal of Tissue Engineering Research
基金
2012年度内蒙古自治区高等学校研究项目(NJZY12151)~~
关键词
组织构建
组织构建与生物活性因子
酸性成纤维细胞生长因子
受体
细胞外调节蛋白激酶
缺血-再灌注损伤
小肠
小肠绒毛上皮细胞
其他基金
组织构建图片文章
tissue construction
tissue construction and bioactive factor
acidic fibroblast growth factor
receptor
extracellular signal-regulated kinase
ischemia-reperfusion injury
small intestine
intestinal villus epithelial cells
other grants-supported paper
tissue construction photographs-containing paper
