摘要
目的:探讨大黄素甲醚对脑缺血再灌注后IL-1β含量和ICAM-1及caspase-3表达的影响。方法:91只SD大鼠随机分为正常组(normal),假手术组(sham),模型组(model),大黄素甲醚大剂量(PHD)及小剂量(PLD)组。采用线栓法复制大鼠右侧大脑中动脉脑缺血再灌注模型,用放射免疫法测定病变侧脑组织IL-1β的含量,用免疫组织化学方法测定ICAM-1和caspase-3表达的变化,并进行组织病理学观察。结果:Model组再灌注6h病变侧IL-1β含量明显升高且达高峰,再灌注24h病变侧ICAM-1、caspase-3表达明显升高,中性粒细胞附壁浸润明显;大黄素甲醚PHD组再灌注12h、24h病变侧IL-1β、ICAM-1和caspase-3表达明显低于model组相应时段(P<0.05或P<0.01),中性粒细胞附壁浸润较少。结论:大黄素甲醚可降低脑缺血再灌注后IL-1β、ICAM-1和caspase-3水平,减轻脑缺血再灌注损伤。
AIM： To explore the effect of physcion （P） on the level of 1L - 1 β and expression of ICAM - 1 and easpase - 3 duting cerebral ischemia - repeffusion injury. METHODS： The 91 healthy adult SD rats were selected, and were randomly divided into normal group, sham- operated group, cerebral ischemia- repeffusion group （model）, low- dose physcion （PLD） and high- dose physcion （PHD） treatment group. The level of IL- 1β was detected by radioimmunoassay. The expression of ICAM - 1 and caspase- 3 was detected by immunohistochemistry. The changes of tissue pathology were also investigated. RESULTS： The level of 1L - 1β reached the peak at 6 h after ischemia - reperfusion （ IR）. The protein expression of ICAM - 1 and caspase - 3 reached the peak at 24 h after 1R. The level of IL- 1β and the protein expression of 1CAM- 1 and caspase- 3 in PHD group decreased obviously compared with those in model group （ P 〈 0.05 or P 〈 0.01 ）, infiltration and adhesiveness of neutrophils were less serious at the same time. CONCLUSION： Physcion decreases the level of IL- 1β and the protein expression of ICAM - 1 and caspase - 3 to protect brain tissue from cerebral ischemia- reperfusion injury.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第9期1829-1833,共5页
Chinese Journal of Pathophysiology
